Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2450:467-478.
doi: 10.1007/978-1-0716-2172-1_24.

Monitoring Telomere Maintenance During Regeneration of Annelids

Nithila A Joseph  1 , Chi-Fan Chen  2 , Jiun-Hong Chen  3 , Liuh-Yow Chen  4
Affiliations

Monitoring Telomere Maintenance During Regeneration of Annelids

Nithila A Joseph et al. Methods Mol Biol. 2022.

Abstract

Telomere shortening is a hallmark of aging and eventually constrains the proliferative capacity of cells. The protocols discussed here are used for monitoring telomeres comprehensively in Aeolosoma viride, a model system for regeneration studies. We present methods for analyzing the activity of telomerase enzyme in regenerating tissue by telomeric repeat amplification protocol (TRAP) assay, for comparing telomere length between existing tissue and newly regenerated tissue by telomere restriction fragment (TRF) assay, as well as for visualizing telomeres by fluorescence in situ hybridization (FISH).

Keywords: Aeolosoma viride; Regeneration; TRAP assay; TRF assay; Telomere FISH; Telomere maintenance.

PubMed Disclaimer

References

    1. Baguna J, Salo E, Auladell C (1989) Regeneration and pattern formation in planarians III. Evidence that neoblasts are totipotent stem cells and the source of blastema cells. Development 107:77–86
    1. Michalopoulos GK (2007) Liver regeneration. J Cell Physiol 213:286–300. https://doi.org/10.1002/jcp.21172 doi: 10.1002/jcp.21172. - DOI - PMC - PubMed
    1. Li Q, Yang H, Zhong TP (2015) Regeneration across metazoan phylogeny: lessons from model organisms. J Genet Genomics 42:57–70. https://doi.org/10.1016/j.jgg.2014.12.002 doi: 10.1016/j.jgg.2014.12.002. - DOI - PubMed
    1. Tsonis PA, Madhavan M, Tancous EE, Del Rio-Tsonis K (2004) A newt’s eye view of lens regeneration. Int J Dev Biol 48:975–980. https://doi.org/10.1387/ijdb.041867pt doi: 10.1387/ijdb.041867pt. - DOI - PubMed
    1. Watson JD (1972) Origin of concatemeric T7 DNA. Nat New Biol 239(94):197–201. https://doi.org/10.1038/newbio239197a0 doi: 10.1038/newbio239197a0. - DOI - PubMed

LinkOut - more resources