Single-neuron projectome of mouse prefrontal cortex

Le Gao^#^{1

2}, Sang Liu^#^{1

2

3}, Lingfeng Gou^#¹, Yachuang Hu^#^{1

2}, Yanhe Liu^{1

2}, Li Deng¹, Danyi Ma^{1

2}, Haifang Wang¹, Qiaoqiao Yang⁴, Zhaoqin Chen¹, Dechen Liu¹, Shou Qiu^{1

2}, Xiaofei Wang¹, Danying Wang¹, Xinran Wang¹, Biyu Ren¹, Qingxu Liu¹, Tianzhi Chen¹, Xiaoxue Shi¹, Haishan Yao¹, Chun Xu¹, Chengyu T Li¹, Yangang Sun¹, Anan Li^{5

6}, Qingming Luo^{5

6

7}, Hui Gong^{8

9}, Ninglong Xu^{10

11

12}, Jun Yan^{13

14

15}

Affiliations

¹ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
² University of Chinese Academy of Sciences, Shanghai, China.
³ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China.
⁴ Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
⁵ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.
⁶ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China.
⁷ School of Biomedical Engineering, Hainan University, Haikou, China.
⁸ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China. huigong@mail.hust.edu.cn.
⁹ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China. huigong@mail.hust.edu.cn.
¹⁰ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. xunl@ion.ac.cn.
¹¹ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China. xunl@ion.ac.cn.
¹² Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, China. xunl@ion.ac.cn.
¹³ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. junyan@ion.ac.cn.
¹⁴ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China. junyan@ion.ac.cn.
¹⁵ Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, China. junyan@ion.ac.cn.

^# Contributed equally.

PMID: 35361973
DOI: 10.1038/s41593-022-01041-5

Single-neuron projectome of mouse prefrontal cortex

Le Gao et al. Nat Neurosci. 2022 Apr.

. 2022 Apr;25(4):515-529.

doi: 10.1038/s41593-022-01041-5. Epub 2022 Mar 31.

Authors

Affiliations

¹ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
² University of Chinese Academy of Sciences, Shanghai, China.
³ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China.
⁴ Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
⁵ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.
⁶ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China.
⁷ School of Biomedical Engineering, Hainan University, Haikou, China.
⁸ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China. huigong@mail.hust.edu.cn.
⁹ HUST-Suzhou Institute for Brainsmatics, JITRI Institute for Brainsmatics, Suzhou, China. huigong@mail.hust.edu.cn.
¹⁰ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. xunl@ion.ac.cn.
¹¹ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China. xunl@ion.ac.cn.
¹² Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, China. xunl@ion.ac.cn.
¹³ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. junyan@ion.ac.cn.
¹⁴ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China. junyan@ion.ac.cn.
¹⁵ Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, China. junyan@ion.ac.cn.

^# Contributed equally.

PMID: 35361973
DOI: 10.1038/s41593-022-01041-5

Abstract

Prefrontal cortex (PFC) is the cognitive center that integrates and regulates global brain activity. However, the whole-brain organization of PFC axon projections remains poorly understood. Using single-neuron reconstruction of 6,357 mouse PFC projection neurons, we identified 64 projectome-defined subtypes. Each of four previously known major cortico-cortical subnetworks was targeted by a distinct group of PFC subtypes defined by their first-order axon collaterals. Further analysis unraveled topographic rules of soma distribution within PFC, first-order collateral branch point-dependent target selection and terminal arbor distribution-dependent target subdivision. Furthermore, we obtained a high-precision hierarchical map within PFC and three distinct functionally related PFC modules, each enriched with internal recurrent connectivity. Finally, we showed that each transcriptome subtype corresponds to multiple projectome subtypes found in different PFC subregions. Thus, whole-brain single-neuron projectome analysis reveals organization principles of axon projections within and outside PFC and provides the essential basis for elucidating neuronal connectivity underlying diverse PFC functions.

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References

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Single-neuron projectome of mouse prefrontal cortex

Affiliations

Single-neuron projectome of mouse prefrontal cortex

Authors

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Abstract

References

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MeSH terms

LinkOut - more resources

Full Text Sources

Miscellaneous