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. 2022 Jun;28(6):1296-1298.
doi: 10.3201/eid2806.220194. Epub 2022 Apr 1.

Detecting SARS-CoV-2 Omicron B.1.1.529 Variant in Wastewater Samples by Using Nanopore Sequencing

Lasse D Rasmussen, Stine R Richter, Sofie E Midgley, Kristina T Franck

Detecting SARS-CoV-2 Omicron B.1.1.529 Variant in Wastewater Samples by Using Nanopore Sequencing

Lasse D Rasmussen et al. Emerg Infect Dis. 2022 Jun.

Abstract

We report wastewater surveillance for SARS-CoV-2 variants of concern by using mutation-specific, real-time PCR and rapid nanopore sequencing. This surveillance might be useful for an early warning in a scenario in which a new variant is emerging, even in areas that have low virus incidences.

Keywords: B.1.1.529; COVID-19; Denmark. Omicron; SARS-CoV-2; coronaviruses; emerging variants; nanopore sequencing; respiratory infections; severe acute respiratory syndrome coronavirus 2; variants of concern; viruses; wastewater surveillance; zoonoses.

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References

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Supplementary concepts