Spatiotemporal regulation of switching front-rear cell polarity

Abstract

Bacterial cells are spatiotemporally highly organised with proteins localising dynamically to distinct subcellular regions. Motility in the rod-shaped Myxococcus xanthus cells represents an example of signal-induced spatiotemporal regulation of cell polarity. M. xanthus cells move across surfaces with defined front-rear polarity; occasionally, they invert polarity and, in parallel, reverse direction of movement. The polarity module establishes front-rear polarity between reversals and consists of the Ras-like GTPase MglA and its cognate GEF and GAP, that all localise asymmetrically to the cell poles. The Frz chemosensory system constitutes the polarity inversion module and interfaces with the proteins of the polarity module, thereby triggering their polar repositioning. As a result, the polarity proteins, over time, toggle between the cell poles causing cells to oscillate irregularly. Here, we review recent progress in how front-rear polarity is established by the polarity module and inverted by the Frz system and highlight open questions for future studies.

Keywords: Cell polarity; Collective cell migration; Fruiting body formation; G protein; GAP; GEF; Gliding motility; Ras-like GTPase; Spatiotemporal regulation; Twitching motility; Type IV pili.