Purification of nitrogen regulator II, the product of the glnL (ntrB) gene of Escherichia coli
- PMID: 3536843
- PMCID: PMC213583
- DOI: 10.1128/jb.168.2.1002-1004.1986
Purification of nitrogen regulator II, the product of the glnL (ntrB) gene of Escherichia coli
Abstract
We purified the product of glnL (ntrB), NRII, and the product of a mutant glnL allele, NRII2302. In vitro transcription of the nitrogen-regulated promoter glnAp2 by purified components of Escherichia coli required NRII or NRII2302 when the template DNA was linear.
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