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Comparative Study
. 1986 Dec;168(3):1466-7.
doi: 10.1128/jb.168.3.1466-1467.1986.

Determination of bacterial cell volume with the Coulter Counter

Comparative Study

Determination of bacterial cell volume with the Coulter Counter

H E Kubitschek et al. J Bacteriol. 1986 Dec.

Abstract

Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., electronic measurement with a Coulter Counter-Analyzer system and biophysical measurement of the total volume and number of cells in sedimented cell pellets. Within experimental errors, the methods gave the same mean cell volumes.

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References

    1. J Bacteriol. 1986 Nov;168(2):613-8 - PubMed
    1. J Biol Chem. 1977 Nov 10;252(21):7850-61 - PubMed
    1. Biophys J. 1974 Feb;14(2):119-23 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1611-7 - PubMed

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