Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar 24:2022:9252901.
doi: 10.1155/2022/9252901. eCollection 2022.

Potential Lignocellulolytic Microfungi from Pineapple Plantation for Composting Inoculum Additive

Bambang Irawan  1 , Inten Wahyuningtias  1 , Niken Ayuningtyas  1 , Ola Apriyani Isky  1 , Salman Farisi  1 , Sumardi Sumardi  1 , Afandi Afandi  2 , Sutopo Hadi  3
Affiliations

Potential Lignocellulolytic Microfungi from Pineapple Plantation for Composting Inoculum Additive

Bambang Irawan et al. Int J Microbiol. .

Abstract

Pineapple plantations leave a lot of plant biomass after the planting season ends. The abundant residue of pineapple plants causes problems due to the high content of lignocellulose, which is difficult to decompose naturally. This study aimed to isolate and characterize lignocellulolytic microfungi isolates from pineapple plantations. The information of this study was used as data to prepare an inoculum for the induction of pineapple litter composting that was resistant to stress to pineapple plantation habitat. The results showed that there were 11 dominant lignocellulolytic microfungi isolates found from pineapple litter and plantation soil. Using selective media, the selection showed five cellulolytic (Bioggp 3, 6, 9, 11, and 12); five xylanolytic (Bioggp 3, 6, 8, 9, and 12); and two ligninolytic microfungi isolates (Bioggp 2 and 5). Bioggp 3, 6, 9, and 12 are cellulolytic and xylanolytic with Bioggp 3 showing the highest cellulolytic index (4.0) and xylanolytic index (4.20). Testing of ligninolytic microfungi showed that the Bioggp 5 isolate had a stronger lignin indicator (color intensity = 4.0 and zone ratio of 1.47) than the Bioggp 2 isolate. Bioggp 9 had the highest cellulolytic isolate spore productivity at 4.5 × 108 spores/mL with 93.3% spore viability, and Bioggp 3 had the highest xylanolytic isolate spore productivity at 2.5 × 109 spores/mL with 89.3% spore viability. Bioggp 2 had the highest ligninolytic isolate spore productivity at 1.8 × 109 spores/mL, but Bioggp 5 showed the highest spore viability at 98.0%.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Figure 1
Figure 1
Moist chamber method.
Figure 2
Figure 2
(a) Clear zone of cellulolytic (Bioggp 3); (b) xylanolytic (Bioggp 3) isolate; (c) oxidation of guaiacol of ligninolytic (Bioggp 2); and (d) (Bioggp 5) isolate on specific media.
See this image and copyright information in PMC

References

    1. Ali M. M., Hashim N., Aziz S. A., Lasekan O. Pineapple (Ananas comosus): a comprehensive review of nutritional values, volatile compounds, health benefits, and potential food products. Food Research International . 2020;137(109675) - PubMed
    1. Pardo M. E. S., Casselis M. E. S., Mora-Escobedo R., Garcia E. J. Chemical characterisation of the industrial residues of the pineapple (Ananas omosus) Journal of Agricultural Chemistry and Environment . 2014;3(2):53–56. doi: 10.4236/jacen.2014.32b009. - DOI
    1. Gupta P., Samant K., Sahu A. Isolation of cellulosa-degrading bacteria and determination of their cellulolytic potential. International Journal of Microbiology . 2012;2012:6. doi: 10.1155/2012/578925.578925 - DOI - PMC - PubMed
    1. Dahmen N., Lewandowski I., Zibek S., Weidtmann A. Integrated lignocellulosic value chains in a growing bioeconomy: status quo and perspectives. GCB Bioenergy . 2019;11(1):107–117. doi: 10.1111/gcbb.12586. - DOI
    1. Hart T. D., De Leij F. A. A. M., Kinsey G., Kelley J., Lynch J. M. Strategies for the isolation of cellulolytic fungi for composting of wheat straw. World Journal of Microbiology and Biotechnology . 2002;18(5):471–480. doi: 10.1023/a:1015519005814. - DOI

LinkOut - more resources