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Meta-Analysis
. 2022 Mar 17:13:852428.
doi: 10.3389/fendo.2022.852428. eCollection 2022.

Effect of Vitamin D Supplementation on In Vitro Fertilization Outcomes: A Trial Sequential Meta-Analysis of 5 Randomized Controlled Trials

Xiaoting Zhou  1   2   3 , Xiaomei Wu  1   2   3 , Xi Luo  1   2   3   4 , Jingyi Shao  1   2 , Dongqun Guo  5 , Bo Deng  1   2 , Ze Wu  1   2
Affiliations
Meta-Analysis

Effect of Vitamin D Supplementation on In Vitro Fertilization Outcomes: A Trial Sequential Meta-Analysis of 5 Randomized Controlled Trials

Xiaoting Zhou et al. Front Endocrinol (Lausanne). .

Abstract

Despite numerous studies indicating an imperative role of vitamin D for reproduction, the importance of vitamin D supplementation on in vitro fertilization (IVF) outcomes remains controversial. We therefore performed this meta-analysis to investigate the IVF outcomes of vitamin D supplementation in infertile women with vitamin D deficiency. We systematically searched PubMed, Embase and the Cochrane library for identifying all relevant studies published before August 2021. Pregnancy rate was defined as the primary outcome while good quality embryo, fertilization rate, ongoing pregnancy, and miscarriage were secondary outcomes. We used Review Manager 5.3 (RevMan) to conduct meta-analysis and examined the robustness of the primary outcome by trial sequential analysis. Five studies were included in the final analysis and it suggested that vitamin D supplementation was associated with improved chemical pregnancy rate (risk ratio [RR] = 1.53, 95% confidence interval [CI] = 1.06 to 2.20, p = 0.02) but not benefited in improving clinical pregnancy rate (RR = 1.34, 95% CI = 0.81 to 2.24, p = 0.25) and all secondary outcomes. Trial sequential analysis suggested further studies are needed to confirm this conclusion. We concluded that vitamin D supplementation should be prescribed to improve chemical pregnancy in infertile women with vitamin D deficiency and more studies are required to further confirm this finding.

Keywords: in vitro fertilization; meta-analysis; trial sequential analysis; vitamin D deficiency; vitamin D supplementation.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Flow diagram of searching and selecting eligible study in this meta-analysis.
Figure 2
Figure 2
Risk of bias summary of eligible studies.
Figure 3
Figure 3
Meta-analysis of pregnancy rate between vitamin D supplementation and no supplementation.
Figure 4
Figure 4
Trial sequential analysis of chemical and clinical pregnancy. (A) Required sample size of 462; (B) required sample size of 1570.
Figure 5
Figure 5
Meta-analysis of secondary outcomes, namely, good quality embryo (A), fertilization rate (B), ongoing pregnancy (C), and miscarriage (D).
See this image and copyright information in PMC

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References

    1. Inhorn MC, Patrizio P. Infertility Around the Globe: New Thinking on Gender, Reproductive Technologies and Global Movements in the 21st Century. Hum Reprod Update (2015) 21:411–26. doi: 10.1093/humupd/dmv016 - DOI - PubMed
    1. Vander Borght M, Wyns C. Fertility and Infertility: Definition and Epidemiology. Clin Biochem (2018) 62:2–10. doi: 10.1016/j.clinbiochem.2018.03.012 - DOI - PubMed
    1. Chen W, Peng Y, Ma X, Kong S, Tan S, Wei Y, et al. . Integrated Multi-Omics Reveal Epigenomic Disturbance of Assisted Reproductive Technologies in Human Offspring. EBioMedicine (2020) 61:103076. doi: 10.1016/j.ebiom.2020.103076 - DOI - PMC - PubMed
    1. Kamath MS, Mascarenhas M, Franik S, Liu E, Sunkara SK. Clinical Adjuncts in In Vitro Fertilization: A Growing List. Fertil Steril (2019) 112:978–86. doi: 10.1016/j.fertnstert.2019.09.019 - DOI - PubMed
    1. Morshed-Behbahani B, Mossalanejad L, Shahsavari S, Dastpak M. The Experiences of Infertile Women on Assistant Reproductive Treatments: A Phenomenological Study. Iran Red Crescent Med J (2012) 14:382–3. - PMC - PubMed