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. 1986 Jun 20;189(4):701-7.
doi: 10.1016/0022-2836(86)90499-7.

Ultrastructure of native lipoprotein from Escherichia coli envelopes

Ultrastructure of native lipoprotein from Escherichia coli envelopes

D J Manstein et al. J Mol Biol. .

Abstract

The free form of the major lipoprotein from Escherichia coli cells envelopes has been purified to homogeneity by gentle extraction procedures and conventional chromatographic separations in a non-ionic detergent. The morphology of paracrystals obtained from homogeneous protein was investigated by low-dose electron microscopy. Electron diffraction of the paracrystals was consistent with alpha-helices arranged perpendicularly to the main cross-band with a periodicity of 20 nm.

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