Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Nov 25;14(22):8963-77.
doi: 10.1093/nar/14.22.8963.

Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae

E Kellermann, P G Seeboth, C P Hollenberg
Free PMC article

Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae

E Kellermann et al. Nucleic Acids Res. .
Free PMC article

Abstract

The PDC1 gene of Saccharomyces cerevisiae, encoding pyruvate decarboxylase was sequenced. The gene contains an open reading frame of 1647 base pairs. The codon usage shows the same strong bias as found for some other glycolytic enzymes. Transcription starts mainly at -30 and terminates 100 base pairs downstream of the termination codon. In some strains a second termination site, 46 base pairs upstream of the stop codon was observed. The function of the promoter region was analyzed by fusion to the bacterial structural gene encoding beta-lactamase (bla). On multicopy plasmid or integrated in the genome, the expression of the bla gene showed the regulation of the authentic PDC1 gene.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. J Biol Chem. 1984 Nov 10;259(21):13334-43 - PubMed
    1. Biochim Biophys Acta. 1975 Oct 20;405(2):492-9 - PubMed
    1. J Mol Biol. 1978 Dec 25;126(4):813-46 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 - PubMed

Publication types

Associated data