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. 2022 Apr 6;17(4):e0266567.
doi: 10.1371/journal.pone.0266567. eCollection 2022.

A comparative study on the effect of blood collection tubes on stress oxidative markers

Alireza Bastin  1 , Saba Fooladi  2 , Amir Hossein Doustimotlagh  3 , Sina Vakili  4 , Amir Hashem Aminizadeh  2 , Sanaz Faramarz  1 , Hamidreza Shiri  5 , Mohammad Hadi Nematollahi  6   7
Affiliations

A comparative study on the effect of blood collection tubes on stress oxidative markers

Alireza Bastin et al. PLoS One. .

Abstract

Oxidative stress has a major role in disease pathogenesis. However, limited studies have investigated the effect of various sample collection tubes on oxidative biomarkers. The present study aimed to evaluate the effect of different collection tubes on the variation of malondialdehyde (MDA), nitric oxide (NO), total thiol (t-SH), and ferric reducing ability of plasma (FRAP) levels. A total of 35 individuals participated in this study and each collected sample was separated into three different tubes: glass tubes (GTs), plain plastic tubes (PTs), and gel separator tubes (GSTs). The results of PTs and GSTs were compared to those of GTs as the reference tube. The comparison between the means of biomarkers in various tubes indicated that there was no significant difference in MDA results between tubes. In contrast, t-SH and NO content were significantly decreased in GSTs and PTs compared to GTs. However, the Bland-Altman analysis showed an acceptable concordance for the mentioned analytes and the statistically significant differences were not clinically significant for NO, MDA, and t-SH antioxidant parameters. Moreover, the FRAP level was considerably lower in GSTs compared to GTs. Nevertheless, the Bland-Altman analysis showed a high bias percentage for the FRAP assay when using PTs and GSTs. According to the present results, it can be concluded that switching to plastic blood collection tubes or serum separation tubes could influence the FRAP results. However, there was no interference for the interpretation of other antioxidant assays in different types of collection tubes. Hence, it is suggested to use GTs for total antioxidant capacity evaluations, especially the FRAP assay.

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Conflict of interest statement

Authors state no conflict of interest.

Figures

Fig 1
Fig 1. The comparison of mean concentration of (a) total Thiol, (b) NO, (c) MDA, and (d) FRAP levels in GST, GT, and PT.
Wilcoxon matched paired test was used, * < 0.05, ** < 0.01.
Fig 2
Fig 2. The comparison of median, mean, first, and third quartile concentration of (a) total Thiol, (b) NO, (c) MDA, and (d) FRAP in GST, GT, and PT.
Fig 3
Fig 3. Bland-Altman difference plots for the antioxidant parameters obtained with PT, GST and GT tubes.
(a, b) total Thiol, (c, d) NO, (e, f) MDA, (g, h) FRAP values. The dashed lines are the limit of agreements (LOA), which correspond to the mean ± 1.96 SD of the difference between the tubes.
Fig 4
Fig 4. Comparison of assay results after collection of blood in PT, GT, and GST tubes, using passing and bablok regression analysis.
Regression line (full line) with its 95% confidence interval (broken lines). Comparison of results for PT, GST and GT tubes for the antioxidants parameters.
Fig 5
Fig 5. Pearson correlation coefficient (r) values of correlation analysis for the antioxidant parameters obtained with PT, GST and GT tubes.
(a) total Thiol, (b) NO, (c) MDA, and (d) FRAP.
See this image and copyright information in PMC

References

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