Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar 31;12(4):e12136.
doi: 10.1002/clt2.12136. eCollection 2022 Apr.

Recent patents in allergy and immunology: A quantitative real-time polymerase chain reaction method for diagnosing asthma and asthma exacerbation

Pureun-Haneul Lee  1 , SeonMuk Choi  1 , MinHyeok An  1 , An-Soo Jang  1
Affiliations

Recent patents in allergy and immunology: A quantitative real-time polymerase chain reaction method for diagnosing asthma and asthma exacerbation

Pureun-Haneul Lee et al. Clin Transl Allergy. .

Abstract

Background: Asthma is diagnosed based on a history of the characteristic symptoms and evidence of expiratory airflow limitation. However, asthma diagnosis using the existing tests is associated with a risk of coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 spread. In this study, we developed a quantitative real-time polymerase chain reaction (qRT-PCR)-based asthma diagnosis tool.

Methods: We detected nectin-4 in the plasma of patients with asthma using qRT-PCR, explored the relationship of clinical variables.

Results: quantitative real-time polymerase chain reaction revealed that plasma nectin-4 mRNA levels were higher in asthmatics than controls. These results correlated with lung function.

Conclusions: Those results suggest that qRT-PCR for nectin-4 may aid asthma diagnosis and monitoring.

Keywords: asthma; diagnosis; nectin‐4; qRT‐PCR.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interest.

Figures

FIGURE 1
FIGURE 1
(A) Overview of the nectin‐4 quantitative real‐time polymerase chain reaction assay, (B) nectin‐4 mRNA levels in control subjects and asthma patients, and the correlation thereof with lung function
See this image and copyright information in PMC

References

    1. Global Initiative for Asthma . Global Strategy for Asthma Management and Prevention. GINA; 2020. [Internet]. 2021 February 23. http://ginasthma.org/2020‐gina‐report‐global‐strategy‐for‐asthma‐managem...
    1. Celebi Sözener Z, Cevhertas L, Nadeau K, Akdis M, Akdis CA. Environmental factors in epithelial barrier dysfunction. J Allergy Clin Immunol. 2020;145(6):1517‐1528. - PubMed
    1. Julie L, Kirstin E, Nick S. Real‐Time PCR: Current Technology and Applications. Caister Academic Press; 2009.
    1. Volz E, Mishra S, Chand M, et al. Assessing transmissibility of SARS‐CoV‐2 lineage B.1.1.7 in England. Nature. 2021;593(7858):266‐269. - PubMed
    1. Singh BK, Hornick AL, Krishnamurthy S, et al. The nectin‐4/afadin protein complex and intercellular membrane pores contribute to rapid spread of measles virus in primary human airway epithelia. J Virol. 2015;89:7089‐7096. - PMC - PubMed