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Comment
. 2022 Apr 7;386(14):1385-1386.
doi: 10.1056/NEJMc2117480.

Evidence of Artemisinin-Resistant Malaria in Africa

Barbara H Stokes  1 , Kurt E Ward  1 , David A Fidock  1
Affiliations
Comment

Evidence of Artemisinin-Resistant Malaria in Africa

Barbara H Stokes et al. N Engl J Med. .
No abstract available

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Conflict of interest statement

No potential conflict of interest relevant to this letter was reported.

Figures

Figure 1.
Figure 1.. Ring-Stage Parasite Survival in CRISPR-Cas9 Gene–Edited Plasmodium falciparum Lines Expressing kelch13 Mutations Associated with Artemisinin Resistance in Northern Uganda.
The mean survival among ring-stage parasites is plotted as a function of dihydroartemisinin concentration in parental or mutant Dd2 (Panel A) and MAS-136 (Panel B) strains. Error bars indicate the standard error. The strains were assayed in duplicate on six occasions for the Dd2 strains and on four occasions for the MAS-136 strains. Synchronized early ring-stage parasites (0 to 3 hours after the invasion of red cells) were exposed in ring-stage survival assays (RSA0–3h) to dihydroartemisinin for 6 hours, the drug was removed by extensive washing, and cultures were continued for 66 hours. The numbers of live intraerythrocytic parasites were measured by flow cytometry, and survival after drug treatment was calculated relative to dimethylsulfoxide (vehicle control)–treated parasites. Unpaired t-tests with Welch’s correction were used to compare edited kelch13 mutant strains with wild-type unedited parental strains. CRISPR denotes clustered regularly interspaced short palindromic repeats.
See this image and copyright information in PMC

Comment on

  • Evidence of Artemisinin-Resistant Malaria in Africa.
    Balikagala B, Fukuda N, Ikeda M, Katuro OT, Tachibana SI, Yamauchi M, Opio W, Emoto S, Anywar DA, Kimura E, Palacpac NMQ, Odongo-Aginya EI, Ogwang M, Horii T, Mita T. Balikagala B, et al. N Engl J Med. 2021 Sep 23;385(13):1163-1171. doi: 10.1056/NEJMoa2101746. N Engl J Med. 2021. PMID: 34551228

References

    1. Balikagala B, Fukuda N, Ikeda M, et al. Evidence of artemisinin-resistant malaria in Africa. N Engl J Med 2021;385:1163–71. - PubMed
    1. Stokes BH, Dhingra SK, Rubiano K, et al. Plasmodium falciparum K13 mutations in Africa and Asia impact artemisinin resistance and parasite fitness. Elife 2021;10:e66277. - PMC - PubMed