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. 2022 May 19;11(5):e0009522.
doi: 10.1128/mra.00095-22. Epub 2022 Apr 7.

Genome Sequences of Five Arenaviruses from Pygmy Mice (Mus minutoides) in Sierra Leone

Matej Vučak  1 , James Bangura  2 , Bruno M Ghersi  3 , Jenna Nichols  1 , Joseph Hughes  1 , Ana da Silva Filipe  1 , Alexandre Tremeau-Bravard  3 , David J Wolking  3 , Emmanuel Amara  2 , Abdulai Bangura  2 , Marilyn C Kanu  2 , Osman T Kanu  2 , Dickson Kargbo  2 , Edwin G Lavalie  2 , Victor Lungay  2 , Willie Robert  2 , Mohamed Turay  2 , Steven Fornie  4 , Thomas T Samba  4 , Bankolay B Sesay  4 , Patrick Swaray  4 , Mohamed A Vandi  4 , Mohamed Alpha Bah  5 , Andrew A Mansaray  5 , Brian H Bird  3 , Andrew J Davison  1
Affiliations

Genome Sequences of Five Arenaviruses from Pygmy Mice (Mus minutoides) in Sierra Leone

Matej Vučak et al. Microbiol Resour Announc. .

Abstract

The genome sequences of five strains of a mammarenavirus were assembled from metagenomic data from pygmy mice (Mus minutoides) captured in Sierra Leone. The nearest fully sequenced relatives of this virus, which was named Seli virus, are lymphocytic choriomeningitis virus, Lunk virus, and Ryukyu virus.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
Phylogenetic analyses of SELV. The complete amino acid sequences of the L (A) and the NP (B) proteins of Old World mammarenaviruses and partial nucleotide sequences of the L (C) and the NP (D) genes of SELV and its closer relatives were aligned using MAFFT v7.475 (13). The alignments were inspected visually in JalView v2.11 (14). Maximum likelihood phylogenies for each alignment were reconstructed using IQ-Tree v2.1.3 (15) by instituting the best fitting model selected by ModelFinder according to the Bayesian information criterion. The trees in A and B were rooted using a New World mammarenavirus, Junín virus (not shown; GenBank accession no. AY358022 and AY358023, respectively). The trees in C and D were rooted using Lassa virus (not shown; GenBank accession no. U73034 and J04324, respectively). Branch support was assessed using 1,000 bootstrap replicates, with values above 75% shown at the nodes and values of 100% indicated by asterisks. The viruses are denoted by GenBank accession no. and name, and SELV strains are indicated by bold font. The scale bars represent substitutions per site.
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