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. 2022 Jun 10;84(6):760-765.
doi: 10.1292/jvms.21-0508. Epub 2022 Apr 11.

A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA

Kikumi Ogihara  1 , Yuko Naya  1 , Junichi Kamie  2 , Shin Hisamatsu  3 , Michi Kodama  4 , Yoshiharu Ishikawa  4 , Koichi Kadota  4
Affiliations

A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA

Kikumi Ogihara et al. J Vet Med Sci. .

Abstract

A cell line (PL38PB) was established from blood samples of a 6-month-old pig that was diagnosed with lymphoma with CD5 expression. Histopathological examination revealed neoplastic lesions in the spleen, liver and lymph nodes. Tumor cells were immunohistochemically positive for CD20 and immunoglobulin heavy chains (μ, γ and α). Membranous CD5 and cytoplasmic Immunoglobulin M (IgM), ​Immunoglobulin G (IgG) and ​Immunoglobulin A (IgA) were detected in PL38PB cells by flow cytometry. In addition, the cytoplasm of PL38PB cells were positive for IgM, IgG and IgA by immunofluorescent. However, no Ig secretion was detected in culture supernatant by Ouchterlony gel diffusion method. Results suggest that PL38PB cells express three Ig isotypes that are produced but not secreted.

Keywords: CD5; cell line; immunoglobulin; lymphoma; swine.

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Figures

Fig. 1.
Fig. 1.
Cytology. Most white blood cells are larger in size than normal lymphocytes. May-Grünwald-Giemsa. Bar=50 μm.
Fig. 2.
Fig. 2.
Histology and immunohistochemistry. (A) Internal iliac lymph node. Lymphoid cells (arrowheads) and plasmacytoid cells with abundant cytoplasm (arrows) are observed. There are no distinct differences in nuclear morphology between these cells. HE. Bar=5 μm. (B) Internal iliac lymph node. CD5-positive lymphoma cells. Streptavidin-biotin-peroxidase complex (SAB). Bar=5 μm. (C) Spleen. Cluster of plasmacytoid cells show cytoplasmic positivity for α chain. SAB. Bar=5 μm.
Fig. 3.
Fig. 3.
Cytology of PL38PB cells. The cells are spherical and medium- to large-sized. PL38PB cells have a large N/C ratio and a basophilic cytoplasm. The nucleus is round, and some cells have cuts in their nucleus. May-Grünwald-Giemsa. Bar=10 μm.
Fig. 4.
Fig. 4.
Immunohistochemistry of PL38PB cells. (A) The cells show cytoplasmic positivity for λ light chain. Polymer method. Bar=10 μm. (B) Cells are negative for κ light chain. Polymer method. Bar=10 μm.
Fig. 5.
Fig. 5.
Electron microscopy of PL38PB cells. The nuclei are round or slightly irregular, and elongated strands of rough endoplasmic reticulum (arrow) are visible. Bar=2 μm.
Fig. 6.
Fig. 6.
Double immunofluorescent staining of PL38PB cells (A) Green fluorescence indicating the presence of ​Immunoglobulin G (IgG) is observed in the cytoplasm of cultured cells. (B) Red fluorescence for IgM is detected in the same cells as in Fig. 6A. (C) Merged photograph of Fig. 6A and 6B. Bar=10 μm.
Fig. 7.
Fig. 7.
Flow cytometric analysis of PL38PB cells. The ratio of positive cells for Immunoglobulin G (IgG), Immunoglobulin M (IgM), Immunoglobulin A (IgA), CD5, BLA36, CD79b and CD44 are 84.4, 62.1, 68.3, 63.5, 25.3, 18.4 and 76.6, respectively.
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References

    1. Aksungar F. B., Ayer M., Serteser M., Coskun A., Unsal I.2014. A triclonal gammopathy in a relapsing multiple myeloma patient, detected by immunosubtraction method. Ann. Clin. Biochem. 51: 606–610. doi: 10.1177/0004563213512801 - DOI - PubMed
    1. Chan J. K. C.2007. Tumors of the lymphoreticular system. pp. 1139–1288. In: Diagnostic Histopathology of Tumors, 3rd ed. (Fletcher, C. D. M. ed.), Churchill Livingstone, London.
    1. Drexler H. G.2011. Establishment and culture of leukemia-lymphoma cell lines. Methods Mol. Biol. 731: 181–200. doi: 10.1007/978-1-61779-080-5_16 - DOI - PubMed
    1. Dupuis M. M., Tuchman S. A.2016. Non-secretory multiple myeloma: from biology to clinical management. OncoTargets Ther. 9: 7583–7590. doi: 10.2147/OTT.S122241 - DOI - PMC - PubMed
    1. Feller A. C., Diebold J.2004. Variant: Lymphoplasmacytoid lymphoma (immunocytoma). pp. 31–34. In: Histopathology of Nodal and Extranodal Non-Hodgkin’s Lymphomas, 3rd ed., Springer, Berlin.