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Editorial
. 2022 Mar 23:28:168-174.
doi: 10.1016/j.omtn.2022.03.002. eCollection 2022 Jun 14.

Editorial: In vitro mechanistic evaluation of nucleic acid polymers: A cautionary tale

Andrew Vaillant  1
Affiliations
Editorial

Editorial: In vitro mechanistic evaluation of nucleic acid polymers: A cautionary tale

Andrew Vaillant. Mol Ther Nucleic Acids. .
No abstract available

Keywords: hepatitis B; mechanism of action; nucleic acid polymer; oligonucleotide; transfection artifacts.

PubMed Disclaimer

Conflict of interest statement

A.V. is an employee and shareholder of Replicor Inc.

Figures

None
Graphical abstract
Figure 1
Figure 1
Sequence and chemical modifications of various NAPs. All NAPs contain phosphorothioate at each linkage
Figure 2
Figure 2
Effects of pH on NAP structure and antiviral activity against HBV in vitro with and without transfection and in vivo
Figure 3
Figure 3
NAP trafficking and effects on antiviral activity (A) Comparison of oligonucleotide trafficking occurring in vivo and in humans with that occurring in vitro. (B) Artifacts associated with transfection-based approaches with NAPs.
Figure 4
Figure 4
pH can influence the secondary structure of NAPs (A) Secondary structure of NAPs with various sequence compositions under non-denaturing and denaturing gel electrophoresis. (B) Effect of pH on the secondary structure of REP 2107. All NAPs contain phosphorothioate at each linkage. A, adenosine; G, guanosine; T, thymidine; C, cytidine; d, deoxyribose (DNA); 2′OMe, 2′O-methyl-modified ribose (RNA).
Figure 5
Figure 5
Model for the impact of LNA modification on the antiviral interactions of NAPs Hydrophilic regions of target alpha helices for NAPs are indicated in blue and hydrophobic regions in red. Hydrophobic interactions and their relative strength are indicated by arrows.
See this image and copyright information in PMC

References

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