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Editorial
. 2022 Apr;22(4):395-397.
doi: 10.1080/14737159.2022.2065194. Epub 2022 Apr 13.

Transcription activator-like effector nuclease (TALEN) as a promising diagnostic approach for COVID-19

Emad Behboudi  1 , Parisa Zeynali  2 , Vahideh Hamidi-Sofiani  1 , Britt Nakstad  3   4 , Alireza Tahamtan  1   5
Affiliations
Editorial

Transcription activator-like effector nuclease (TALEN) as a promising diagnostic approach for COVID-19

Emad Behboudi et al. Expert Rev Mol Diagn. 2022 Apr.
No abstract available

Keywords: COVID-19; CRISPR; SARS-CoV-2; TALEN; molecular diagnosis.

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Figures

Figure 1.
Figure 1.
Illustration of workflow in Real-time PCR, CRISPR, and TALEN. In Real-time PCR, the trained operators require specific kits and facilities, but in CRISPR, a fragment of interest region of RNA target in isothermal condition and by reverse transcription-recombinase polymerase amplification (RT-RPA), is amplified to DNA and then by T7 transcription converted to RNA. The attachment of Cas13a–CRISPR RNA to amplified target RNAs stimulates the collateral activity of Cas13a, which then cleaves reporters. Separated RNA reporters should be captured on a colorimetric lateral-flow strip (biotin–fluorescein RNA reporter) or visualized by fluorescence signal (fluorescent reporter). A similar procedure is performed in TALEN but with more efficiency.
See this image and copyright information in PMC

References

    1. Teymoori-Rad M, Samadizadeh S, Tabarraei A, et al. Ten challenging questions about SARS-CoV-2 and COVID-19. Expert review of respiratory medicine. Expert Rev Respir Med. 2020;14(9):881–888. - PubMed
    1. Kohmer N, Rabenau HF, Hoehl S, et al. Comparative analysis of point-of-care, high-throughput and laboratory-developed SARS-CoV-2 nucleic acid amplification tests (NATs). J Virol Methods. 2021;291:114102. - PMC - PubMed
    1. Tahamtan A, Ardebili A.. Real-timeRT-PCR in COVID-19 detection: issues affecting the results. Expert review of molecular diagnostics. Expert Rev Mol Diagn. 2020;20(5):453–454. - PMC - PubMed
    1. Sasano Y, et al. CRISPR-PCS: a powerful new approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae. Sci. Rep. 2016;6(1):1–11. - PMC - PubMed
    1. Mohammadinejad R, Biagioni A, Arunkumar G, et al. EMT signaling: potential contribution of CRISPR/Cas gene editing. Cell Mol Life Sci: CMLS. 2020;77(14):2701–2722. - PMC - PubMed

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