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. 2022:2502:161-182.
doi: 10.1007/978-1-0716-2337-4_11.

Analysis of Nuclear Pore Complexes in Caenorhabditis elegans by Live Imaging and Functional Genomics

Patricia de la Cruz Ruiz #  1 , Raquel Romero-Bueno #  1 , Peter Askjaer  2
Affiliations

Analysis of Nuclear Pore Complexes in Caenorhabditis elegans by Live Imaging and Functional Genomics

Patricia de la Cruz Ruiz et al. Methods Mol Biol. 2022.

Abstract

Nuclear pore complexes (NPCs) are essential to communication of macromolecules between the cell nucleus and the surrounding cytoplasm. RNA synthesized in the nucleus is exported through NPCs to function in the cytoplasm, whereas transcription factors and other proteins are selectively and actively imported. In addition, many NPC constituents, known as nuclear pore proteins (nucleoporins or nups), also play critical roles in other processes, such as genome organization, gene expression, and kinetochore function. Thanks to its genetic amenability and transparent body, the nematode Caenorhabditis elegans is an attractive model to study NPC dynamics. We provide here an overview of available genome engineered strains and FLP/Frt-based tools to study tissue-specific functions of individual nucleoporins. We also present protocols for live imaging of fluorescently tagged nucleoporins in intact tissues of embryos, larvae, and adult and for analysis of interactions between nucleoporins and chromatin by DamID.

Keywords: CRISPR-Cas9; Caenorhabditis elegans; DamID; FLP; Live imaging; NPC; Nuclear pore complex; Nucleocytoplasmic transport; Nucleoporin; npp.

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References

    1. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77(1):71–94 - DOI
    1. Sulston JE, Schierenberg E, White JG, Thomson JN (1983) The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev Biol 100(1):64–119. https://doi.org/10.1016/0012-1606(83)90201-4 - DOI - PubMed
    1. Sulston JE, Horvitz HR (1977) Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Dev Biol 56(1):110–156. https://doi.org/10.1016/0012-1606(77)90158-0 - DOI - PubMed
    1. San-Miguel A, Lu H (2013) Microfluidics as a tool for C. elegans research. WormBook 1–19. https://doi.org/10.1895/wormbook.1.162.1
    1. Perez-Jimenez MM, Rodriguez-Palero MJ, Rodenas E, Askjaer P, Munoz MJ (2014) Age-dependent changes of nuclear morphology are uncoupled from longevity in Caenorhabditis elegans IGF/insulin receptor daf-2 mutants. Biogerontology 15(3):279–288. https://doi.org/10.1007/s10522-014-9497-0 - DOI - PubMed

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