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. 2022 Apr 5:18:349-357.
doi: 10.2147/TCRM.S349900. eCollection 2022.

Single Nucleotide Polymorphism in the 3' Untranslated Region of PRKAA2 on Cardiometabolic Parameters in Type 2 Diabetes Mellitus Patients Who Received Metformin

Dita Maria Virginia  1 , Christine Patramurti  2 , Fenty  1   3 , Christianus Heru Setiawan  1 , Jeffry Julianus  2 , Phebe Hendra  1 , Nicholas Adi Perdana Susanto  3
Affiliations

Single Nucleotide Polymorphism in the 3' Untranslated Region of PRKAA2 on Cardiometabolic Parameters in Type 2 Diabetes Mellitus Patients Who Received Metformin

Dita Maria Virginia et al. Ther Clin Risk Manag. .

Abstract

Purpose: This study aimed to explore the association of rs857148 A>C as 3'UTR variants with blood pressure, HbA1c profile, and lipid profiles as cardiometabolic parameters among patients with T2DM receiving metformin.

Patients and methods: This cross-sectional analytic research was conducted with 114 consecutively selected patients with T2DM. Polymerase chain reaction-restriction fragment length polymorphism was conducted to determine rs857148. A total of 108 patients fulfilled inclusion and exclusion criteria.

Results: Genotype distribution agreed with the Hardy Weinberg Equation for Equilibrium (p>0.05) but wildtype allele was found as the minor allele. Subjects with CC genotype and C allele had enhanced HbA1c levels (OR=7.12; 95% CI=1.05-48.26; p=0.04; OR=1.66; 95% CI=1.06-2.60; p=0.03, respectively). It was confirmed by dominant model whereas subjects with AA tended to have reduced HbA1c compared to AC+CC genotype (OR=0.15; 95% CI=0.02-0.97; p=0.047). AC genotype had significant correlation to total cholesterol (OR=1.05; 95% CI=1.01-1.10; p=0.03) compared to AA genotype.

Conclusion: We conclude that polymorphism of rs87148, specifically CC genotype and C allele, has a significant association with HbA1c and total cholesterol after considering oral hypoglycemia agent dose, age, gender, and combination therapy, compared to AA genotype. Future studies that involve a larger sample population and more rigorous selection criteria are required.

Keywords: 3ʹUTR; PRKAA2; cardiometabolic; rs857148; type 2 diabetes mellitus.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
The PCR-RFLP results to determine PRKAA2 rs857148 variants. L= ladder (marker 50 bp), 8, 9, 10, 11, 13 are mutant homozygote genotype, 12 is wildtype homozygote genotype, and 14 is heterozygote genotype.
None
See this image and copyright information in PMC

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