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. 2021 Oct 23:3:100047.
doi: 10.1016/j.fochms.2021.100047. eCollection 2021 Dec 30.

Enzymatic hydrolysis and microbial fermentation: The most favorable biotechnological methods for the release of bioactive peptides

Dora Elisa Cruz-Casas  1 , Cristóbal N Aguilar  1 , Juan A Ascacio-Valdés  1 , Raúl Rodríguez-Herrera  1 , Mónica L Chávez-González  1 , Adriana C Flores-Gallegos  1
Affiliations

Enzymatic hydrolysis and microbial fermentation: The most favorable biotechnological methods for the release of bioactive peptides

Dora Elisa Cruz-Casas et al. Food Chem (Oxf). .

Abstract

Bioactive peptides are biomolecules derived from proteins. They contain anywhere from 2 to 20 amino acids and have different bioactivities. For example, they have antihypertensive activity, antioxidant activity, antimicrobial activity, etc. However, bioactive peptides are encrypted and inactive in the parental protein, so it is necessary to release them to show their bioactivity. For this, there are different methods, where biotechnological methods are highly favorable, highlighting enzymatic hydrolysis and microbial fermentation. The choice of the method to be used depends on different factors, which is why it is essential to know about the process, its principle, and its advantages and disadvantages. The process of peptide release is critical to generate various peptide sequences, which will produce different biological effects in the hydrolysate. This review focuses on providing extensive information on the enzymatic method and microbial fermentation to facilitate selecting the method that provides the most benefits.

Keywords: Bioactive peptides; Biotechnology; Enzymatic hydrolysis; Microbial fermentation.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Diagram of the different methods used for the release of encrypted bioactive peptides.
Fig. 2
Fig. 2
Graphic representation of the enzymatic hydrolysis process in the release of bioactive peptides.
See this image and copyright information in PMC

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