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. 2022 Jul;49(7):717-729.
doi: 10.1111/jcpe.13629. Epub 2022 May 3.

Pasteurized Akkermansia muciniphila reduces periodontal and systemic inflammation induced by Porphyromonas gingivalis in lean and obese mice

Hannah Mulhall  1 , Jeanne M DiChiara  2 , Olivier Huck  3   4 , Salomon Amar  1   2
Affiliations

Pasteurized Akkermansia muciniphila reduces periodontal and systemic inflammation induced by Porphyromonas gingivalis in lean and obese mice

Hannah Mulhall et al. J Clin Periodontol. 2022 Jul.

Abstract

Aim: The aim of this study was to evaluate the effect of the administration of pasteurized Akkermansia muciniphila and Amuc_1100 on periodontal destruction in lean and obese mice and to determine the impact of the mode of administration.

Materials and methods: Porphyromonas gingivalis-associated experimental periodontitis was induced in lean and obese mice. After 3 weeks, live, pasteurized A. muciniphila or Amuc_1100 was administered by oral or gastric gavage for three additional weeks. Moreover, an evaluation of the interaction between A. muciniphila and P. gingivalis was performed by RNA-sequencing, and cytokines secretion was measured in exposed macrophages.

Results: Oral administration of live, pasteurized A. muciniphila or Amuc_1100 significantly decreased P. gingivalis-induced periodontal destruction and inflammatory infiltrate in lean and obese mice and contributed to the reduction of the plasma level of TNF-α and to the increase of IL-10. The co-culture of A. muciniphila and P. gingivalis induced an increased expression of genes linked to the synthesis of monobactam-related antibiotics in A. muciniphila, while a decrease of the gingipains and type IX secretion system was observed in P. gingivalis. In P. gingivalis-infected macrophages, pasteurized A. muciniphila decreased TNF-α and increased IL-10 levels.

Conclusions: Pasteurized A. muciniphila can counteract P. gingivalis-associated periodontal destruction.

Keywords: gut; obesity; periodontal treatment; periodontitis; probiotic.

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References

REFERENCES

    1. Batool, F., Morand, D. N., Thomas, L., Bugueno, I. M., Aragon, J., Irusta, S., Keller, L., Benkirane-Jessel, N., Tenenbaum, H., & Huck, O. (2018). Synthesis of a novel electrospun polycaprolactone scaffold functionalized with ibuprofen for periodontal regeneration: An in vitro andIn vivo study. Materials (Basel), 11(4), 580. https://doi.org/10.3390/ma11040580
    1. Bouaziz, W., Davideau, J. L., Tenenbaum, H., & Huck, O. (2015). Adiposity measurements and non-surgical periodontal therapy outcomes. Journal of Periodontology, 86(9), 1030-1037. https://doi.org/10.1902/jop.2015.140734
    1. Coretti, L., Cuomo, M., Florio, E., Palumbo, D., Keller, S., Pero, R., Chiariotti, L., Lembo, F., & Cafiero, C. (2017). Subgingival dysbiosis in smoker and nonsmoker patients with chronic periodontitis. Molecular Medicine Reports, 15(4), 2007-2014. https://doi.org/10.3892/mmr.2017.6269
    1. Dao, M. C., Everard, A., Aron-Wisnewsky, J., Sokolovska, N., Prifti, E., Verger, E. O., Kayser, B. D., Levenez, F., Chilloux, J., Hoyles, L., Consortium, M. I.-O., Dumas, M. E., Rizkalla, S. W., Dore, J., Cani, P. D., & Clement, K. (2016). Akkermansia muciniphila and improved metabolic health during a dietary intervention in obesity: Relationship with gut microbiome richness and ecology. Gut, 65(3), 426-436. https://doi.org/10.1136/gutjnl-2014-308778
    1. Depommier, C., Everard, A., Druart, C., Plovier, H., Van Hul, M., Vieira-Silva, S., Falony, G., Raes, J., Maiter, D., Delzenne, N. M., de Barsy, M., Loumaye, A., Hermans, M. P., Thissen, J. P., de Vos, W. M., & Cani, P. D. (2019). Supplementation with Akkermansia muciniphila in overweight and obese human volunteers: A proof-of-concept exploratory study. Nature Medicine, 25(7), 1096-1103. https://doi.org/10.1038/s41591-019-0495-2

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