Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jan 8;11(4):2167-2174.
doi: 10.1039/d0ra06665f. eCollection 2021 Jan 6.

Sensitive impedimetric detection of troponin I with metal-organic framework composite electrode

Arushi Gupta  1   2 , Sandeep Kumar Sharma  3 , Vivek Pachauri  4 , Sven Ingebrandt  4 , Suman Singh  1 , Amit L Sharma  1   2 , Akash Deep  1   2
Affiliations

Sensitive impedimetric detection of troponin I with metal-organic framework composite electrode

Arushi Gupta et al. RSC Adv. .

Abstract

Metal-organic frameworks (MOFs) are promising materials for biosensing applications due to their large surface to volume ratio, easy assembly as thin films, and better biocompatibility than other nanomaterials. Their application in electrochemical biosensing devices can be realized by integrating them with other conducting materials, like polyaniline (PANI). In the present research, a composite of a copper-MOF (i.e., Cu3(BTC)2) with PANI has been explored to develop an impedimetric sensor for cardiac marker troponin I (cTnI). The solvothermally synthesized Cu3(BTC)2/PANI composite has been coated as a thin layer on the screen-printed carbon electrodes (SPE). This electroconductive thin film was conjugated with anti-cTnI antibodies. The above formed immunosensor has allowed the impedimetric detection of cTnI antigen over a clinically important concentration range of 1-400 ng mL-1. The whole process of antigen analysis could be completed within 5 min. The detection method was specific to cTnI even in the co-presence of other possibly interfering proteins.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Fig. 1
Fig. 1. (a) FTIR spectra of Cu3(BTC)2, Cu3(BTC)2/PANI, and Ab/Cu3(BTC)2/PANI; (b) XRD patterns of Cu3(BTC)2 and Cu3(BTC)2/PANI.
Fig. 2
Fig. 2. FE-SEM of (a) Cu3(BTC)2; (b) Cu3(BTC)2/PANI.
Fig. 3
Fig. 3. (a) TEM image of Cu3(BTC)2/PANI; (b) N2 adsorption–desorption isotherms for Cu3(BTC)2 and Cu3(BTC)2/PANI.
Fig. 4
Fig. 4. Nyquist plots recorded for Cu3(BTC)2, Cu3(BTC)2/PANI, and Ab/Cu3(BTC)2/PANI electrodes during different stages of biosensor preparation. Experimental and fitted Nyquist plots are shown as dot and line curves, respectively.
Fig. 5
Fig. 5. (a) EIS response of Ab/Cu3(BTC)2/PANI biosensor toward different concentration of cTnI (1–400 ng mL−1); (b) corresponding calibration plot between Rct values and cTnI concentration; (c) experimental and fitted Nyquist plots for the response of Ab/Cu3(BTC)2/PANI sensor towards 400 ng mL−1 and 200 ng mL−1 cTnI; (d) experimental and fitted Nyquist plots for the response of Ab/Cu3(BTC)2/PANI sensor towards 100 ng mL−1, 10 ng mL−1, and 1 ng mL−1 cTnI.
Fig. 6
Fig. 6. (a) Response time of Ab/Cu3(BTC)2/PANI toward 10 ng mL−1 cTnI; (b) response of different batches of prepared Ab/Cu3(BTC)2/PANI biosensor toward 10 ng mL−1 cTnI.
Fig. 7
Fig. 7. Variation in the Rct of Ab/Cu3(BTC)2/PANI biosensor against cTnI and some other non-specific analytes (cTnI = 400 ng mL−1, BSA and ferritin = 500 ng mL−1).
See this image and copyright information in PMC

References

    1. Sun D. Luo Z. Lu J. Zhang S. Che T. Chen Z. et al. Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA nanotetrahedron structure. Biosens. Bioelectron. 2019;134:49–56. doi: 10.1016/j.bios.2019.03.049. - DOI - PubMed
    1. Han X. Li S. Peng Z. Othman A. M. Leblanc R. Recent development of cardiac troponin I detection. ACS Sens. 2016;1(2):106–114.
    1. Zhang G.-J. Luo Z. H. H. Huang M. J. Kang T. G. Ji H. An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood. Biosens. Bioelectron. 2011;28(1):459–463. doi: 10.1016/j.bios.2011.07.007. - DOI - PubMed
    1. Huang K.-J. Niu D.-J. Sun J.-Y. Han C.-H. Wu Z.-W. Li Y.-L. et al. Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA. Colloids Surf., B. 2011;82(2):543–549. doi: 10.1016/j.colsurfb.2010.10.014. - DOI - PubMed
    1. Tuteja S. K. Kukkar M. Suri C. Paul A. Deep A. One step in situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI. Biosens. Bioelectron. 2015;66:129–135. doi: 10.1016/j.bios.2014.10.072. - DOI - PubMed