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. 1987 Feb;61(2):633-7.
doi: 10.1128/JVI.61.2.633-637.1987.

Expression of the art gene protein of human T-lymphotropic virus type III (HTLV-III/LAV) in bacteria

W C Goh, J G Sodroski, C A Rosen, W A Haseltine

Expression of the art gene protein of human T-lymphotropic virus type III (HTLV-III/LAV) in bacteria

W C Goh et al. J Virol. 1987 Feb.

Abstract

Human T-lymphotropic virus type III (HTLV-III/LAV or HIV) contains a gene designated art (anti-repressor transactivator). Here, we report the expression of the art gene product in bacteria and show that the 20-kilodalton (kDa) bacterially expressed art protein is recognized by serum of a patient. The bacterially synthesized art protein competed in an immunological reaction with a 20-kDa protein produced in HTLV-III/LAV-infected lymphocytes. Antiserum to a synthetic oligopeptide corresponding to a sequence in the second exon of the art gene also precipitated the 20-kDa protein in HTLV-III/LAV-infected cells. These results demonstrate that the 20-kDa art gene product is expressed in cell lines that produce HTLV-III/LAV virions.

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References

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