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. 2022 Feb 18;7(2):584-591.
doi: 10.1002/lio2.757. eCollection 2022 Apr.

Expression of circadian clock genes in leukocytes of patients with Meniere's disease

Chao-Hui Yang  1 , Chung-Feng Hwang  1 , Nai-Wen Tsai  2 , Ming-Yu Yang  1   3
Affiliations

Expression of circadian clock genes in leukocytes of patients with Meniere's disease

Chao-Hui Yang et al. Laryngoscope Investig Otolaryngol. .

Abstract

Objectives: The underlying etiology of Meniere's disease (MD) is not completely clear, but the precipitated triggers may alter the circadian clock in patients with MD. This study aims to survey the expression of circadian clock genes in peripheral blood (PB) leukocytes of MD patients.

Methods: We investigated the expression of nine circadian clock genes in the PB leukocytes of patients with MD and normal controls using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).

Results: We observed significantly lower expression of PER1 gene and higher expression of CLOCK gene in MD patients than those in normal controls (p < 0.05). PER1 did not associate with the degree of dizziness handicap in the patients with MD, but a lower expression of PER1 was significantly correlated with higher pure tone average (PTA) and speech reception threshold of the affected ear (p < 0.05). Patients with PTA > 30 dB had significantly lower PER1 expression than those with PTA ≤30 dB in the affected ear (p < 0.05). Our qRT-PCR result was validated by fewer positively stained leukocytes for PER1 protein in the MD patients using the immunocytochemical study.

Conclusion: Our study implies the alteration of the circadian clock in patients with MD. In particular, the downregulation of PER1 correlated with the degree of hearing loss in the affected ear. PER1 in PB leukocytes may be a potential marker for the progression of hearing loss in MD.

Keywords: Meniere's disease; PER1; circadian clock; hearing loss.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIGURE 1
FIGURE 1
The expression of nine circadian clock genes in patients with MD and controls. Data are mean ± SE of –ΔC. *p < 0.05, ***p < 0.001
FIGURE 2
FIGURE 2
The expression of nine circadian clock genes in patients with MD with different degrees of dizziness handicap (A) and hearing loss (B). Data are median and interquartile range of –ΔCt. **p < 0.01
FIGURE 3
FIGURE 3
Representative image and quantification of immunocytochemical analysis for PER1 in PB leukocytes. Most cells were positively stained (brown) for PER1 in the control group, whereas only a few cells were stained for PER1 in the MD group. Data are median and interquartile range of PER1‐positive cells (%). **p < 0.01
See this image and copyright information in PMC

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