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. 2022 Apr 13:10:e13278.
doi: 10.7717/peerj.13278. eCollection 2022.

Identifying and characterizing Stagonosporopsis cucurbitacearum causing spot blight on Pinellia ternata in China

Jia Zhou  1 , Jiawei Xu  1 , Rong Xu  1 , Qiaohuan Chen  1 , Yunhan Wang  1 , Bisheng Huang  1 , Dahui Liu  1 , Yuhuan Miao  1
Affiliations

Identifying and characterizing Stagonosporopsis cucurbitacearum causing spot blight on Pinellia ternata in China

Jia Zhou et al. PeerJ. .

Abstract

Background: Pinellia ternata (Thunb.), a perennial herbal plant in the Araceae family, has great medicinal value and market demand. In August 2020, an outbreak of severe leaf spot blight disease resulted in a huge yield loss of P. ternata. It is necessary to isolate and identify the pathogens that cause spot blight on P. ternata.

Methods: In this study, we isolated and identified the pathogens by fulfilling Koch's postulates. Disease samples with typical spot blight symptoms were collected and pathogens were isolated from the diseased tissues. The pathogen was identified based on its biological characteristics and molecular analysis of internal transcribed (rDNA-ITS) and large subunit (LSU) sequences. Phylogenetic tree were constructed using MEGA7 software and pathogenicity tests were performed using in vivo inoculation. Finally, the pathogen was recovered and identified from the inoculated plants.

Results: Based on Koch's postulates, we identified the pathogen causing spot blight on P. ternata as Stagonosporopsis cucurbitacearum. To our knowledge, this is the first study to explore spot blight on P. ternata caused by S. cucurbitacearum in China.

Keywords: Fungal pathogen; Pinellia ternata; Spot blight; Stagonosporopsis cucurbitacearum.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1. Spot blight disease of P. ternata in field and Pathogenicity test in live plants.
(A and B) The phenotype of P. ternata spot blight disease in field. (C) Disease symptoms of P. ternata seedling at 7 days post inoculation with AG-3. (D) Disease symptoms of P. ternata seedling at 15 days post inoculation with AG-3.
Figure 2
Figure 2. The morphology of AG-3 colony.
(A) The morphological characteristics of seven-day-old colony of AG-3 on PDA. (B) Twenty-day-old colony of AG-3 on PDA. (C) The morphological characters of conidia (100× magnification, dyeing treatment by cotton blue). (D) The features of chlamydospores chain of AG-3 (100× magnification, dyeing treatment by cotton blue).
Figure 3
Figure 3. A maximum parsimony phylogeny tree for Stagonosporopsis sp.
Phylogenetic tree constructed with sequences of internal transcribed spacer ribosomal DNA (rDNA) region (ITS) of isolates AG-3 obtained in this study and other species retrieved from GenBank. The tree was constructed using the neighbor-joining method from the alignment of ITS sequences using MEGA software. Isolates from P. ternata are marked in bold.
See this image and copyright information in PMC

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