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. 2022 Jun;36(6):e24439.
doi: 10.1002/jcla.24439. Epub 2022 Apr 20.

The correlation of lncRNA SNHG16 with inflammatory cytokines, adhesion molecules, disease severity, and prognosis in acute ischemic stroke patients

Chen Xie  1 , Bin Zhu  2 , Juxian Gu  3 , Muhua Sun  4
Affiliations

The correlation of lncRNA SNHG16 with inflammatory cytokines, adhesion molecules, disease severity, and prognosis in acute ischemic stroke patients

Chen Xie et al. J Clin Lab Anal. 2022 Jun.

Abstract

Background: Long non-coding RNA small nucleolar RNA host gene 16 (lncRNA SNHG16) is involved in the pathogenesis of acute ischemic stroke (AIS) through the regulation of brain endothelial cell viability, inflammation, atherosclerotic plaque formation, and neural apoptosis. This study aimed to evaluate the prognostic value of lncRNA SNHG16 in AIS patients.

Methods: Newly diagnosed AIS patients (N = 120) were serially recruited. Their lncRNA SNHG16 expressions in peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR); serum inflammatory cytokines and adhesion molecules were determined using enzyme-linked immunosorbent assay (ELISA). The accumulating recurrence-free survival (RFS) and overall survival (OS) were analyzed. Moreover, controls (N = 60) were recruited and their lncRNA SNHG16 expressions in PBMCs were detected.

Results: LncRNA SNHG16 was declined in AIS patients compared to controls (p < 0.001). Moreover, lncRNA SNHG16 was not related to any comorbidities in AIS patients (all p > 0.05). Interestingly, lncRNA SNHG16 was negatively related to tumor necrosis factor alpha (TNF-α) (p < 0.001), interleukin 6 (IL-6) (p = 0.013), and intracellular cell adhesion molecule-1 (ICAM-1) (p = 0.024), while positively correlated with interleukin 10 (IL-10) (p = 0.022) in AIS patients. Besides, lncRNA SNHG16 was inversely associated with the National Institutes of Health Stroke Scale (NIHSS) score in AIS patients (p = 0.003). During the follow-up period, in 14 (11.7%) patients occurred recurrence and 5 (4.2%) patients died. Unexpectedly, lncRNA SNHG16 was not associated with accumulating RFS (p = 0.103) or OS (p = 0.150) in AIS patients.

Conclusion: LncRNA SNHG16 relates to lower inflammatory cytokines, adhesion molecules, and milder disease severity, but fails to predict prognosis in AIS patients.

Keywords: acute ischemic stroke; disease severity; inflammatory cytokines; long non-coding RNA small nucleolar RNA host gene 16; prognosis.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Long non‐coding RNA small nucleolar RNA host gene 16 (LncRNA SNHG16) was reduced in acute ischemic stroke (AIS) patients
FIGURE 2
FIGURE 2
Long non‐coding RNA small nucleolar RNA host gene 16 (LncRNA SNHG16) was linked to inflammatory cytokines and adhesion molecules in acute ischemic stroke (AIS) patients. Association of lncRNA SNHG16 with tumor necrosis factor alpha (TNF‐α) (A), interleukin‐1 beta (IL‐1β) (B), interleukin 6 (IL‐6) (C), interleukin 10 (IL‐10) (D), intracellular cell adhesion molecule‐1 (ICAM‐1) (E), and vascular cell adhesion molecule‐1 (VCAM‐1) (F)
FIGURE 3
FIGURE 3
Long non‐coding RNA small nucleolar RNA host gene 16 (LncRNA SNHG16) was negatively related to disease severity in acute ischemic stroke (AIS) patients
FIGURE 4
FIGURE 4
Long non‐coding RNA small nucleolar RNA host gene 16 (LncRNA SNHG16) high was not associated with accumulating recurrence‐free survival (RFS) in acute ischemic stroke (AIS) patients
FIGURE 5
FIGURE 5
Long non‐coding RNA small nucleolar RNA host gene 16 (LncRNA SNHG16) high was not correlated with accumulating overall survival (OS) in acute ischemic stroke (AIS) patients
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