Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jul;16(7):643-652.
doi: 10.1002/term.3305. Epub 2022 Apr 20.

Electrical stimulation promotes the wound-healing properties of diabetic human skin fibroblasts

Atieh Abedin-Do  1   2 , Ze Zhang  2 , Yvan Douville  2 , Mireille Méthot  2 , Julien Bernatchez  2 , Mahmoud Rouabhia  1
Affiliations

Electrical stimulation promotes the wound-healing properties of diabetic human skin fibroblasts

Atieh Abedin-Do et al. J Tissue Eng Regen Med. 2022 Jul.

Abstract

This study evaluated the effect of low (20 and 40 mV/mm) intensities of electrical stimulation on the proliferation and migration of skin fibroblasts from diabetic donors. We also examined the effect of electrical stimulation on modulating the capacity of fibroblasts to contract collagen gel, express alpha-smooth muscle actin, and secrete proteolytic enzymes involved in regulating extracellular matrix synthesis and degradation. Our study shows that 20 and 40 mV/mm of stimulation increased the growth of fibroblasts extracted from diabetic patients but not from non-diabetic donors. Electrical stimulation increased the migration of diabetic fibroblasts, their capacity to contract collagen gel, and the expression of alpha-smooth muscle actin and promoted different proteolytic enzymes involved in accelerating wound healing. Overall results confirm the effectiveness of electrical stimulation in modulating the wound healing activities of fibroblasts extracted from diabetic skin donors. This study, therefore, suggests the possible use of electrical stimulation to promote diabetic foot ulcer healing by stimulating the wound healing properties of skin fibroblasts.

Keywords: diabetic foot ulcer; electrical stimulation; skin fibroblasts; zymography.

PubMed Disclaimer

References

REFERENCES

    1. Abedin-Do, A., Zhang, Z., Douville, Y., Méthot, M., & Rouabhia, M. (2021). Effect of electrical stimulation on diabetic human skin fibroblast growth and the secretion of cytokines and growth factors involved in wound healing. Biology, 10(7), 641. https://doi.org/10.3390/biology10070641
    1. Almadani, Y. H., Vorstenbosch, J., Davison, P. G., & Murphy, A. M. (2021). Wound healing: A comprehensive review. Seminars in Plastic Surgery, 35(3), 141-144. https://doi.org/10.1055/s-0041-1731791
    1. Al-Rikabi, A. H. A., Tobin, D. J., Riches-Suman, K., & Thornton, M. J. (2021). Dermal fibroblasts cultured from donors with type 2 diabetes mellitus retain an epigenetic memory associated with poor wound healing responses. Scientific Reports, 11(1), 1474. https://doi.org/10.1038/s41598-020-80072-z
    1. Amălinei, C., Căruntu, I. D., & Bălan, R. A. (2007). Biology of metalloproteinases. Romanian Journal of Morphology and Embryology, 48(4), 323-334.
    1. Armstrong, D. G., Boulton, A. J. M., & Bus, S. A. (2017). Diabetic foot ulcers and their recurrence. New England Journal of Medicine, 376, 2367-2375. https://doi.org/10.1056/NEJMra1615439

Publication types

Grants and funding

LinkOut - more resources