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. 2022 Jul;287(1):32-44.
doi: 10.1111/jmi.13107. Epub 2022 Apr 29.

Poly-L-lysine/hyaluronan nanocarriers as a novel nanosystem for gene delivery

Masoumeh Souri  1   2   3 , Mohammad Aref Bagherzadeh  4 , Mirza Ali Mofazzal Jahromi  5   6   7 , Hossein Mohammad-Beigi  8 , Amir Abdoli  9   10 , Hamed Mir  11 , Abazar Roustazadeh  11 , Majid Pirestani  12 , Parham Sahandi Zangabad  3 , Jafar Kiani  13   14 , Amirmahmoud Bakhshayesh  15 , Mehdi Jahani  15   16 , Mohammad Taghi Joghataei  1   17 , Mahdi Karimi  1   2   3
Affiliations

Poly-L-lysine/hyaluronan nanocarriers as a novel nanosystem for gene delivery

Masoumeh Souri et al. J Microsc. 2022 Jul.

Abstract

The present research comes up with a novel DNA-loaded poly-L-lysine (PLL)/hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) were used to analyse the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of -24 ± 3 mV, with an average size of 138 ± 6 nm, in an ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore, the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimised conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.

Keywords: gene delivery; hyaluronan; nanocarriers; poly-L-lysine.

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References

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