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. 2022 Mar 24:13:1.
doi: 10.4103/genint.genint_1_22. eCollection 2022.

Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index

Alkhansa S Mahmoud  1 , Ammar M E Hassan  1 , Amna A Ali  1 , Nuha M Hassan  1 , Amna A Yousif  1 , Fawzia E Elbashir  2 , Ahmed Omer  3 , Omer M Abdalla  1   4
Affiliations

Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index

Alkhansa S Mahmoud et al. Genome Integr. .

Abstract

Radiotherapy is one of the most important options for treating breast cancer in humans. The development of biomarkers to monitor radiosensitivity is scarce. The aim of this study is to investigate the γH2AX levels in the human blood samples 0.5 h after radiotherapy compared to the levels before radiotherapy in breast cancer patients in relation to their respective body mass index (BMI). Blood plasma samples were collected from a total of 20 breast cancer patients before and after radiotherapy to measure γH2AX levels with an antibody against γH2AX based on enzyme-linked immunosorbent assay technique. The median BMI of the patients was 30 kg/m2. γH2AX was differentially expressed in breast cancer patients before radiotherapy. γH2AX levels significantly increased in 14 patients after radiotherapy (P = 0.006), whereas γH2AX levels decreased in three patients after radiotherapy, and three patients were excluded. There was no correlation between γH2AX values after radiotherapy and BMI (P = 0.5, r = 0.1). Our results suggest that γH2AX can be used by ELISA technique to measure γH2AX in the blood plasma of breast cancer patients undergoing radiotherapy and can be considered a biomarker of radiosensitivity.

Keywords: Body mass index; DNA damage; H2AX; radiotherapy.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Mean levels of breast cancer patients showed increased γH2AX levels postradiotherapy (P = 0.006)
Figure 2
Figure 2
Correlation between body mass index and γH2AX (P = 0.5, r = 0.1)
See this image and copyright information in PMC

References

    1. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136:359–86. - PubMed
    1. Rosenblatt E, Izewska J, Anacak Y, Pynda Y, Scalliet P, Boniol M, et al. Radiotherapy capacity in European countries: An analysis of the Directory of Radiotherapy Centres (DIRAC) database. Lancet Oncol. 2013;14:e79–86. - PubMed
    1. Wang W. Radiotherapy in the management of early breast cancer. J Med Radiat Sci. 2013;60:40–6. - PMC - PubMed
    1. Chua ML, Rothkamm K. Biomarkers of radiation exposure: Can they predict normal tissue radiosensitivity? Clin Oncol (R Coll Radiol) 2013;25:610–6. - PubMed
    1. Forker LJ, Choudhury A, Kiltie AE. Biomarkers of tumour radiosensitivity and predicting benefit from radiotherapy. Clin Oncol (R Coll Radiol) 2015;27:561–9. - PubMed

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