Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jun 1:237:113518.
doi: 10.1016/j.ecoenv.2022.113518. Epub 2022 Apr 18.

Effects of acute ammonia nitrogen exposure on metabolic and immunological responses in the Hong Kong oyster Crassostrea hongkongensis

Affiliations
Free article

Effects of acute ammonia nitrogen exposure on metabolic and immunological responses in the Hong Kong oyster Crassostrea hongkongensis

Jie Lu et al. Ecotoxicol Environ Saf. .
Free article

Abstract

Ammonia nitrogen, a major oxygen-consuming pollutant in the environment, can adversely affect aquatic organisms such as fish, bivalves, and crustaceans. We investigated the toxic effects of ammonia nitrogen on the Hong Kong oyster, Crassostrea hongkongensis, using flow cytometry and 1H nuclear magnetic resonance metabolomics. Exposure to ammonia nitrogen caused time- and concentration-dependent alterations in various immune parameters in hemocytes and impaired the metabolic profiles of the gills. We observed changes in the rate of apoptosis, esterase activity, lysosomal mass, hemocyte counts, phagocytic activity, and mitochondrial mass. Exposure affected metabolic pathways involved in energy metabolism, osmotic balance, and oxidative stress. We concluded that ammonia nitrogen induces metabolic and hematological dysfunction in C. hongkongensis, and our findings provide insights into the biochemical defense strategies of bivalves exposed to acute high-concentration ammonia nitrogen.

Keywords: Ammonia nitrogen; Cellular immunity; Crassostrea hongkongensis; Gill; Hemocyte; Metabolomics.

PubMed Disclaimer

LinkOut - more resources