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. 2022 Apr 15;11(8):1083.
doi: 10.3390/plants11081083.

Cytotoxicity Evaluation and Antioxidant Activity of a Novel Drink Based on Roasted Avocado Seed Powder

Andreea Pușcaș  1 , Anda E Tanislav  1 , Romina A Marc  1 , Vlad Mureșan  1 , Andruța E Mureșan  1 , Emoke Pall  2 , Constantin Cerbu  2
Affiliations

Cytotoxicity Evaluation and Antioxidant Activity of a Novel Drink Based on Roasted Avocado Seed Powder

Andreea Pușcaș et al. Plants (Basel). .

Abstract

The avocado seed is an underused waste resulting from the processing of pulp. Polyphenols, fibers, and carotenoids are present in the seed, which also exhibits prophylactic, fungicidal, and larvicidal effects. Developing food products with avocado seed as an ingredient or spice is highly desired for nutritional, environmental, and economic reasons. The present study proposed its valorization in a hot drink, similar to already existing coffee alternatives, obtained by infusing the roasted and grinded avocado seed. The proximate composition of the raw or conditioned avocado seed and that of the novel drink were determined. The total phenolic content was assessed using the Folin-Ciocâlteu method. The total carotenoids were extracted and assessed spectrophotometrically. Starch determination was performed by the Ewers Polarimetric method. The highest content of polyphenols, 772.90 mg GAE/100 g, was determined in the crude seed, while in the drink was as low as 17.55 mg GAE/100 g. However, the proposed drink demonstrated high antioxidant capacity, evaluated through the DPPH method. This might be due to the high content of the total carotenoid compounds determined in the roasted seed (6534.48 µg/100 g). The proposed drink demonstrated high antiproliferative activity on Hs27 and DLD-1 cell lines.

Keywords: antioxidant capacity; avocado seed; bioactive compounds; coffee alternative; cytotoxicity; food waste; valorization.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Antioxidant capacity as measured through DPPH assay; identical superscript letters indicate no significant difference (p > 0.05).
Figure 2
Figure 2
Cell viability of Hs27 cells when treated with the novel roasted avocado seed-based drink.
Figure 3
Figure 3
Cell viability of DLD-1 cells when treated with the novel roasted avocado seed-based drink.
See this image and copyright information in PMC

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