[Enzymuria]

Abstract

Study of enzymuria rises more and more interest in human pathology as a diagnosis parameter of renal diseases or as an index of nephrotoxicity. In this two fields, the literature demonstrated the interest of enzymes of the brush border of proximal tubuli (alanine amino peptidase, gamma glutamyl transpeptidase, alkaline phosphatase) and of lysosomal enzymes (beta glucuronidase and N acetyl beta D glucosaminidase). The authors analyse the problems limiting the present use of these methods: incomplete knowledge of enzyme of the different parts of the renal tissue and of the mechanisms of enzymuria (choice of enzymes and time periods for sampling); analytical problems referring to the study of enzymes in a complex medium (treatment and storage of samples, choice of adapted methods for activity measurement); at last the way of expression the results is still to be defined.