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Review
. 1986:20:465-99.
doi: 10.1146/annurev.ge.20.120186.002341.

Germ-line transformation of mice

R D Palmiter, R L Brinster
Review

Germ-line transformation of mice

R D Palmiter et al. Annu Rev Genet. 1986.
No abstract available

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Figures

Figure 1
Figure 1
Methods of producing transgenic mice that have been devised or proposed. The most popular approach involves direct microinjection of a few hundred copies of linearized DNA into one of the pronuclei of a fertilized egg. DNA has also been injected into the cytoplasm, nuclei of two-cell eggs, or into the blastocoel cavity. Another strategy involves introducing DNA into totipotent teratocarcinoma cells and then mixing some of these cells with normal blastocyst cells to produce a chimeric mouse or using their nuclei to replace the pronuclei of fertilized eggs. Genetically engineered retroviruses are also being developed that carry foreign genes and can infect early embryos or tissue culture cells.
See this image and copyright information in PMC

References

Literature Cited

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    1. Bradley A, Evans M, Kaufman MH, Robertson E. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cells. Nature. 1984;309:255–58. - PubMed
    1. Brand AH, Breeden L, Abraham J, Stemglanz R, Nasmyth K. Characterization of a “silencer“ in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer. Cell. 1985;41:41–48. - PubMed

Note added in proof The following papers were published during the last few months. The titles reveal several exciting developments

    1. Bierberich C, Scangos G, Tanaka K, Jay G. Regulated expression of a murine class I gene in transgenic mice. Mol Cell Biol. 1986:61339–42. - PMC - PubMed
    1. Costantini F, Chada K, Magram J. Correction of murine β-thalassemia by gene transfer into the germ line. Science. 1986;233:1192–94. - PubMed
    1. Kollias G, Wrighton N, Hurst J, Grosveld F. Regulated expression of human Aγ-, β-, and hybrid γβ-globin genes in transgenic mice: manipulation of the developmental expression patterns. Cell. 1986;46:89–94. - PubMed
    1. Leder A, Pattengale PK, Kuo A, Stewart T, Leder P. Consequences of widespread deregulation of the c-myc gene in transgenic mice: multiple neoplasms and normal development. Cell. 1986;45:485–95. - PubMed
    1. Morello D, Moore G, Salmon AM, Yaniv M, Babinet C. Studies on the expression of an H-2K/human growth hormone fusion gene in giant transgenic mice. EMBO J. 1986;5:1877–83. - PMC - PubMed

Publication types

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