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. 2022 Apr 14;12(8):1024.
doi: 10.3390/ani12081024.

A Comparison of Copromicroscopic and Molecular Methods for the Diagnosis of Cat Aelurostrongylosis

Affiliations

A Comparison of Copromicroscopic and Molecular Methods for the Diagnosis of Cat Aelurostrongylosis

Simone Morelli et al. Animals (Basel). .

Abstract

The gold standard method for the diagnosis of cat aelurostrongylosis is the detection of Aelurostrongylus abstrusus first stage larvae with the Baermann's examination. Nevertheless, molecular assays have shown higher diagnostic performances compared to copromicroscopy. This study evaluated the usefulness of an A. abstrusus species-specific PCR on different biological samples collected in clinical settings from 100 privately-owned cats in Italy (n. 60) and Greece (n. 40). A fecal sample was collected from each animal and a pharyngeal swab was also obtained for cats from Italy. All stool samples were subjected to flotation and Baermann's test. The cats were categorized in three groups based on the results of copromicroscopy, i.e., Group A (n. 50 cats with A. abstrusus infection regardless of positivity for other helminths), Group B (n. 25 cats negative for A. abstrusus but positive for at least one of any other helminth), Group C (n. 25 cats negative for any helminth). DNA was extracted from individual aliquots of feces, flotation supernatant, Baermann's sediment and the pharyngeal swab and then subjected to a PCR specific for A. abstrusus. At least one fecal aliquot or the pharyngeal swab scored positive by the A. abstrusus-specific PCR for 48/50 (96%) cats enrolled in Group A; in particular, 38/50 (76%), 35/50 (70%), 41/50 (82%) and 21/25 (84%) DNA extracts from feces, flotation supernatant, Baermann's sediment and pharyngeal swabs were positive by PCR. These results confirm that molecular tools are highly sensitive and specific and indicate that pharyngeal swabs are the most suitable sample for molecular analysis in clinical settings.

Keywords: Baermann; PCR; aelurostrongylosis; cat; diagnosis; molecular; pharyngeal swab.

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Conflict of interest statement

A.M., R.C. and M.B. are IDEXX employees. The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Study sites and number of cats enrolled in the study for each geographic area.

References

    1. Traversa D., Morelli S., Di Cesare A., Diakou A. Felid Cardiopulmonary nematodes: Dilemmas solved and new questions posed. Pathogens. 2021;10:30. doi: 10.3390/pathogens10010030. - DOI - PMC - PubMed
    1. Morelli S., Diakou A., Colombo M., Di Cesare A., Barlaam A., Dimzas D., Traversa D. Cat respiratory nematodes: Current knowledge, novel data and warranted studies on clinical features, treatment and control. Pathogens. 2021;10:454. doi: 10.3390/pathogens10040454. - DOI - PMC - PubMed
    1. Hobmaier M., Hobmaier A. Zur Entwicklung Des Lungenwurmes Der Katze Aelurostrongylus Abstrusus. Z. Parasitenkd. 1935;7:717–718. doi: 10.1007/BF02121331. - DOI
    1. Gerichter C. Studies on the nematodes parasitic in the lungs of felidae in Palestine. Parasitology. 1949;39:251–262. doi: 10.1017/S0031182000083827. - DOI - PubMed
    1. Mackerras M. Observations on the life history of the cat Lungworm. Aelurostrongylus abstrusus (Railliet, 1898) (Nematoda: Metastrongylidae) Aust. J. Zool. 1957;5:188–195.

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