Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Apr 15;23(8):4372.
doi: 10.3390/ijms23084372.

Comment on Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426

Eugene V Sheval  1   2
Affiliations

Comment on Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426

Eugene V Sheval. Int J Mol Sci. .

Abstract

With great interest, I have read the article "Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence" written by Piña et al. [...].

PubMed Disclaimer

Conflict of interest statement

The author declares no conflict of interest.

Comment on

  • Int J Mol Sci. 23:1426.

References

    1. Piña R., Santos-Díaz A.I., Orta-Salazar E., Aguilar-Vazquez A.R., Mantellero C.A., Acosta-Galeana I., Estrada-Mondragon A., Prior-Gonzalez M., Martinez-Cruz J.I., Rosas-Arellano A. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022;23:1426. doi: 10.3390/ijms23031426. - DOI - PMC - PubMed
    1. Sheval E.V., Polzikov M.A., Olson M.O.J., Zatsepina O.V. A Higher Concentration of an Antigen within the Nucleolus May Prevent Its Proper Recognition by Specific Antibodies. Eur. J. Histochem. 2005;49:117–123. - PubMed
    1. Svistunova D.M., Musinova Y.R., Polyakov V.Y., Sheval E.V. A Simple Method for the Immunocytochemical Detection of Proteins inside Nuclear Structures That Are Inaccessible to Specific Antibodies. J. Histochem. Cytochem. 2012;60:152–158. doi: 10.1369/0022155411429704. - DOI - PMC - PubMed
    1. Earley L.F., Kawano Y., Adachi K., Sun X.-X., Dai M.-S., Nakai H. Identification and Characterization of Nuclear and Nucleolar Localization Signals in the Adeno-Associated Virus Serotype 2 Assembly-Activating Protein. J. Virol. 2015;89:3038–3048. doi: 10.1128/JVI.03125-14. - DOI - PMC - PubMed
    1. Miyamoto S., Nakano M., Morikawa T., Hirabayashi A., Tamura R., Fujita-Fujiharu Y., Hirose N., Muramoto Y., Noda T., Fearns R. Migration of Influenza Virus Nucleoprotein into the Nucleolus Is Essential for Ribonucleoprotein Complex Formation. mBio. 2022;13:e03315–e03321. doi: 10.1128/mbio.03315-21. - DOI

MeSH terms

LinkOut - more resources