Reply to Sheval, E.V. Comment on "Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426"

Affiliations

¹ Departamento de Biología, Facultad de Ciencias Básicas, Universidad Metropolitana de Ciencias de la Educación, Santiago 7760197, Chile.
² División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
³ Unidad de Imagenología, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.

Review

Ricardo Piña et al. Int J Mol Sci. 2022.

. 2022 Apr 15;23(8):4375.

doi: 10.3390/ijms23084375.

¹ Departamento de Biología, Facultad de Ciencias Básicas, Universidad Metropolitana de Ciencias de la Educación, Santiago 7760197, Chile.
² División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
³ Unidad de Imagenología, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.

Abstract

We have carefully read the interesting explanatory comment by Eugene V. Sheval [...].

The authors declare no conflict of interest.

1. Sheval E.V. Comment on Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426. Int. J. Mol. Sci. 2022;23:4372. doi: 10.3390/ijms23084372. - DOI - PMC - PubMed
1. Piña R., Santos-Díaz A.I., Orta-Salazar E., Aguilar-Vazquez A.R., Mantellero C.A., Acosta-Galeana I., Estrada-Mondragon A., Prior-Gonzalez M., Martinez-Cruz J.I., Rosas-Arellano A. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022;23:1426. doi: 10.3390/ijms23031426. - DOI - PMC - PubMed
1. Svistunova D.M., Musinova Y.R., Polyakov V.Y., Sheval E.V. A simple method for the immunocytochemical detection of proteins inside nuclear structures that are inaccessible to specific antibodies. J. Histochem. Cytochem. 2012;60:152–158. doi: 10.1369/0022155411429704. - DOI - PMC - PubMed
1. Valencia-Guerrero A., Deng A., Dresser K., Bouliane G., Cornejo K.M. The Value of Direct Immunofluorescence on Proteinase-Digested Formalin-Fixed Paraffin-Embedded Skin Biopsies. Am. J. Dermatopathol. 2018;40:111–117. doi: 10.1097/DAD.0000000000000934. - DOI - PubMed
1. Tian B., Huang W., Huang J., Jiang X., Qin L. Investigation of protease-mediated cuticle-degradation of nematodes by using an improved immunofluorescence-localization method. J. Invertebr. Pathol. 2009;101:143–146. doi: 10.1016/j.jip.2009.05.001. - DOI - PubMed