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. 2022:2504:231-239.
doi: 10.1007/978-1-0716-2341-1_17.

Extracellular Vesicle Loading Via pH-Gradient Modification

Stephanie M Kronstadt  1 , Steven M Jay  1   2 , Anjana Jeyaram  3
Affiliations

Extracellular Vesicle Loading Via pH-Gradient Modification

Stephanie M Kronstadt et al. Methods Mol Biol. 2022.

Abstract

Extracellular vesicles (EVs) have emerged as key mediators of intercellular communication and consequently have the potential to be potent therapeutic vectors. Beyond their endogenous function, EVs are also being harnessed as drug delivery vehicles with possible benefits over synthetic nanoparticle systems. Despite advances in loading exogenous molecules into extracellular vesicles, efficient incorporation of nucleic acids remains a challenge due to aggregation and degradation. In this chapter, we detail a method to load EVs with negatively charged cargo, in particular nucleic acids, by modifying the internal pH of the vesicles to be acidic. This approach demonstrates that pH modification of EVs enables efficient loading of nucleic acids with functional cargo.

Keywords: Exosome; Gene delivery; miRNA; pH-gradient; siRNA.

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References

    1. Camussi G, Deregibus MC, Bruno S et al (2010) Exosomes/microvesicles as a mechanism of cell-to-cell communication. Kidney Int 78(9):838–848 - DOI
    1. Maia J, Caja S, Strano Moraes MC et al (2018) Exosome-based cell-cell communication in the tumor microenvironment. Front Cell Dev Biol 6:18 - DOI
    1. Saffari M, Moghimi HR, Dass CR (2016) Barriers to liposomal gene delivery: from application site to the target. Iran J Pharm Res 15(Suppl):3–17 - PubMed - PMC
    1. Armstrong JPK, Stevens MM (2018) Strategic design of extracellular vesicle drug delivery systems. Adv Drug Deliv Rev 130:12–16 - DOI
    1. Murphy DE, de Jong OG, Evers MJW et al (2021) Natural or synthetic RNA delivery: a stoichiometric comparison of extracellular vesicles and synthetic nanoparticles. Nano Lett 21(4):1888–1895 - DOI

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