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. 2022 Jun:150-151:105164.
doi: 10.1016/j.jcv.2022.105164. Epub 2022 Apr 18.

Evaluation of the respiratory NeuMoDx™ Flu A-B/RSV/SARS-CoV-2 Vantage and Alinity m Resp-4-Plex assays

Mikayla Quinton  1 , Melissa Geahr  1 , Linda Gluck  1 , Junko Jarrett  1 , Heba H Mostafa  2
Affiliations

Evaluation of the respiratory NeuMoDx™ Flu A-B/RSV/SARS-CoV-2 Vantage and Alinity m Resp-4-Plex assays

Mikayla Quinton et al. J Clin Virol. 2022 Jun.

Abstract

Background: December 2021 witnessed an unprecedented increase in SARS-CoV-2 infections in addition to the circulation of influenza A and respiratory syncytial viruses (RSV). Due to increased testing demands for SARS-CoV-2, influenza, and RSV associated with the overall increase in symptomatic respiratory infections, there is an urgent need for multiplex, automated, and high throughput assays in the diagnostic laboratories.

Methods: We compared the performance of the NeuMoDx™ Flu A-B/RSV/SARS-CoV-2 Vantage and the Alinity m Resp-4-Plex to the standard of care influenza A, B, RSV, and SARS-CoV-2 assays used at the Johns Hopkins Microbiology Laboratory. A total of 181 remnant nasopharyngeal swab (NPS) specimens positive for influenza A (n = 29), influenza B (n = 34), RSV (n = 40), SARS-CoV-2 (n = 33), influenza A/RSV (n = 1), and negatives (n = 44) were tested by either or both assays.

Results: Both the NeuMoDx™ Flu A-B/RSV/SARS-CoV-2 Vantage and the Alinity m Resp-4-Plex assays showed 100% total agreement for all the tested analytes. For samples with available cycle threshold (Ct) values, comparable ranges were noted for all targets between the two assays and to the standard of care Ct values as well.

Conclusion: The NeuMoDx™ Flu A-B/RSV/SARS-CoV-2 Vantage and the Alinity m Resp-4-Plex assays showed high sensitivity and accuracy for all the analytes included in both tests. Implementing these assays will assist the diagnostic laboratories with the surge of testing during the 2021-2022 influenza season.

Keywords: Alinity; Flu; NeuMoDx; RSV; SARS-CoV-2.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig 1
Fig. 1
Correlation of the NeuMoDx™ Flu A-B/RSV/SARS-CoV-2 Vantage and Alinity m Resp-4-Plex assays cycle threshold (Ct) values with the standard of care (SOC) test Ct values for SARS-CoV-2, influenza A, influenza B, and RSV.
See this image and copyright information in PMC

References

    1. Uhteg K., Amadi A., Forman M., Mostafa H.H. Circulation of non- SARS-CoV-2 respiratory pathogens and coinfection with SARS-CoV-2 amid the COVID-19 pandemic. Open Forum Infect. Dis. 2021 doi: 10.1093/ofid/ofab618. - DOI - PMC - PubMed
    1. Fall A., Eldesouki R.E., Sachithanandham J., Paul Morris C., Norton J.M., Gaston D.C., Forman M., Abdullah O., Gallagher N., Li M., Swanson N.J., Pekosz A., Klein E.Y., Mostafa H.H. 2022. A quick displacement of the SARS-CoV-2 variant delta with omicron: unprecedented spike in COVID-19 cases associated with fewer admissions and comparable upper respiratory viral loads. medRxiv doi:10.1101/2022.01.26.22269927.
    1. Mostafa H.H., Carroll K.C., Hicken R., Berry G.J., Manji R., Smith E., Rakeman J.L., Fowler R.C., Leelawong M., Butler-Wu S.M., Quintero D., Umali-Wilcox M., Kwiatkowski R.W., Persing D.H., Weir F., Loeffelholz M.J. Multi-center evaluation of the Cepheid Xpert(R) Xpress SARS-CoV-2/Flu/RSV test. J. Clin. Microbiol. 2020 doi: 10.1128/JCM.02955-20. - DOI - PMC - PubMed
    1. Leung EC-m, Chow VC-y, Lee MK-p, Tang KP-s, Li DK-c, Lai RW-m, Hayden R. Evaluation of the Xpert Xpress SARS-CoV-2/Flu/RSV assay for simultaneous detection of SARS-CoV-2, influenza A and B viruses, and respiratory syncytial virus in nasopharyngeal specimens. J. Clin. Microbiol. 2021;59 e02965-20. - PMC - PubMed
    1. Loeffelholz M.J., Alland D., Butler-Wu S.M., Pandey U., Perno C.F., Nava A., Carroll K.C., Mostafa H., Davies E., McEwan A., Rakeman J.L., Fowler R.C., Pawlotsky J.M., Fourati S., Banik S., Banada P.P., Swaminathan S., Chakravorty S., Kwiatkowski R.W., Chu V.C., Kop J., Gaur R., Sin M.L.Y., Nguyen D., Singh S., Zhang N., Persing D.H. Multicenter evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test. J. Clin. Microbiol. 2020;58 - PMC - PubMed