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. 2022 Apr 16:2022:5863952.
doi: 10.1155/2022/5863952. eCollection 2022.

Arbutin Alleviates LPS Induced Sepsis Pneumonia in Mice

Xiang-Xiang Bian  1 , Xuan Zhao  1 , Chun-Hua Ma  2 , Chuan-Pu Shen  1
Affiliations

Arbutin Alleviates LPS Induced Sepsis Pneumonia in Mice

Xiang-Xiang Bian et al. Evid Based Complement Alternat Med. .

Abstract

The aim of this study was to investigate the effects of arbutin (AR) on lipopolysaccharide (LPS)-induced sepsis pneumonia. LPS-induced mice and A549 cells were used to establish septic pneumonia model. AR significantly decreased lung wet-to-dry weight (W/D) ratio, lung myeloperoxidase (MPO) activity and ameliorated lung histopathological changes. In addition, AR increased super oxide dismutase (SOD) activity, decreased malondialdehyde (MDA) content and levels of cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-β) and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) in mice. Furthermore, the results demonstrated that AR inhibited the JAK2/STAT3/NF-κB pathway in LPS-induced A549 cells which was further confirmed by siRNA JAK2 experiment. The experimental results indicated that the protective mechanism of AR on sepsis pneumonia might be attributed partly to the inhibition of cytokine production and JAK2/STAT3/NF-κB pathway.

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Conflict of interest statement

All authors have no conflict of interest.

Figures

Figure 1
Figure 1
The effects of AR on MPO in lung tissue in ALI mice (n = 10). MPO is detected by commercial kit, which is strictly in accordance with the kit instructions. Values are expressed as means ± SD. Compared with control: ##P < 0.01; Compared with LPS: ∗∗P < 0.01.
Figure 2
Figure 2
The effects of AR on W/D in ALI mice (n = 10). The result of dividing the wet weight of the lung tissue by the dry weight is the wet to dry weight ratio of the lung tissue of the mouse. Values are expressed as means ± SD. Compared with control: ##P < 0.01; Compared with LPS: ∗∗P < 0.01.
Figure 3
Figure 3
The effects of AR on SOD activity and MDA content in BALF (a) and cell supernatant (b) (n = 10). SOD and MDA are detected by commercial kit, and the method is according to the kit instructions. Values are expressed as means ± SD. Compared with control: ##P < 0.01; Compared with LPS: ∗∗P < 0.01.
Figure 4
Figure 4
The effects of AR on TNF-α, IL-6 and IL-1β in BALF (a) and cell supernatant (b) (n = 10). TNF-α, IL-6 and IL-1β were detected by commercial kits, and the operation method was carried out according to the kit instructions. Values are expressed as means ± SD. Compared with control: ##P < 0.01; Compared with LPS: ∗∗P < 0.01.
Figure 5
Figure 5
HE staining (x200) (n = 6). Values are expressed as means ± SD. Compared with control: ##P < 0.01; Compared with LPS: ∗∗P < 0.01. White arrows indicate inflammatory infiltration, the red arrow indicates lung atrophy, blue arrow indicates pulmonary edema.
Figure 6
Figure 6
The effects of AR on JAK2/STAT3/NF-κB pathway in mice (a) and A549 cells (b) (n = 10). JAK2/STAT3/NF-κB pathway was detected by western blot. Values are expressed as means ± SD. Compared with control: ##P < 0.01; Compared with LPS: ∗∗P < 0.01.
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