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. 2022 Jul:120:132-134.
doi: 10.1016/j.ijid.2022.04.036. Epub 2022 Apr 25.

Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Meng Yee Lai  1 , Fatma Diyana Mohd Bukhari  1 , Nur Zulaikha Zulkefli  1 , Ilyiana Ismail  2 , Nur Izati Mustapa  2 , Tuan Suhaila Tuan Soh  2 , Afifah Haji Hassan  2 , Kalaiarasu M Peariasamy  3 , Yee Leng Lee  4 , Jeyanthi Suppiah  5 , Ravindran Thayan  5 , Yee Ling Lau  6
Affiliations

Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Meng Yee Lai et al. Int J Infect Dis. 2022 Jul.

Erratum in

Abstract

Objectives: Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix.

Methods: RNA was extracted from nasopharyngeal swab samples by a simple RNA extraction method.

Results: Testing of 77 samples demonstrated 91.2% sensitivity (95% confidence interval [CI]: 78-98.2%) and 100% specificity (95% confidence interval: 92-100%) using UDG RT-LAMP.

Conclusion: This colorimetric UDG RT-LAMP is a simple-to-use, fast, and easy-to-interpret method, which could serve as an alternative for diagnosis of SARS-CoV-2 infection, especially in remote hospitals and laboratories with under-equipped medical facilities.

Keywords: Carryover contamination; RT-LAMP; SARS-CoV-2; UDG; dUTP.

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Conflict of interest statement

Declaration of competing interest The authors have no competing interests to declare.

Figures

Figure 1
Figure 1
Visualization of the RT-LAMP with the color change of phenol red from pink (negative) to yellow (positive). Tube 1: positive reaction; Tube 2: negative reaction. RT-LAMP, reverse transcription loop-mediated isothermal amplification.
See this image and copyright information in PMC

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Supplementary concepts