Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Apr 27;21(1):72.
doi: 10.1186/s12934-022-01799-0.

Engineered probiotics

Junheng Ma #  1   2 , Yuhong Lyu #  1 , Xin Liu  3 , Xu Jia  2   4 , Fangyun Cui  5 , Xiaoheng Wu  1   2 , Shanshan Deng  6 , Changwu Yue  7
Affiliations
Review

Engineered probiotics

Junheng Ma et al. Microb Cell Fact. .

Erratum in

  • Correction to: Engineered probiotics.
    Ma J, Lyu Y, Liu X, Jia X, Cui F, Wu X, Deng S, Yue C. Ma J, et al. Microb Cell Fact. 2022 May 24;21(1):93. doi: 10.1186/s12934-022-01820-6. Microb Cell Fact. 2022. PMID: 35610719 Free PMC article. No abstract available.

Abstract

Engineered probiotics are a kind of new microorganisms produced by modifying original probiotics through gene editing. With the continuous development of tools and technology progresses, engineering renovation of probiotics are becoming more diverse and more feasible. In the past few years there have been some advances in the development of engineered probiotics that will benefit humankind. This review briefly introduces the theoretical basis of gene editing technology and focuses on some recent engineered probiotics researches, including inflammatory bowel disease, bacterial infection, tumor and metabolic diseases. It is hoped that it can provide help for the further development of genetically modified microorganisms, stimulate the potential of engineered probiotics to treat intractable diseases, and provide new ideas for the diagnosis of some diseases or some industrial production.

Keywords: CRISPR; Disease diagnosis and treatment; Engineered probiotics; Gene editing; Genetic engineering; Synthetic biology.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

References

    1. Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. Expert consensus document The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11(8):506–514. doi: 10.1038/nrgastro.2014.66. - DOI - PubMed
    1. Binda S, Hill C, Johansen E, Obis D, Pot B, Sanders ME, et al. Criteria to Qualify Microorganisms as "Probiotic" in Foods and Dietary Supplements. Front Microbiol. 2020;11:1662. doi: 10.3389/fmicb.2020.01662. - DOI - PMC - PubMed
    1. Suez J, Zmora N, Segal E, Elinav E. The pros, cons, and many unknowns of probiotics. Nat Med. 2019;25(5):716–729. doi: 10.1038/s41591-019-0439-x. - DOI - PubMed
    1. O'Toole PW, Marchesi JR, Hill C. Next-generation probiotics: the spectrum from probiotics to live biotherapeutics. Nat Microbiol. 2017;2:17057. doi: 10.1038/nmicrobiol.2017.57. - DOI - PubMed
    1. Chen K, Zhu Y, Zhang Y, Hamza T, Yu H, Saint Fleur A, et al. A probiotic yeast-based immunotherapy against Clostridioides difficile infection. Sci Transl Med. 2020;12:567. - PMC - PubMed