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. 2022 Mar 22;3(2):101300.
doi: 10.1016/j.xpro.2022.101300. eCollection 2022 Jun 17.

Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics

Robert Page  1 , Edward Scourfield  2 , Mattia Ficarelli  2 , Stuart W McKellar  2 , Kwok Leung Lee  2 , Thomas J A Maguire  2 , Clement Bouton  2 , Maria Jose Lista  2 , Stuart J D Neil  2 , Michael H Malim  2 , Mark Zuckerman  3 , Hannah E Mischo  2 , Rocio T Martinez-Nunez  2
Affiliations

Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics

Robert Page et al. STAR Protoc. .

Abstract

The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022).

Keywords: Immunology; Microbiology; Molecular Biology.

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Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
Resuspension of GM pellet by tapping against a tube rack
Figure 2
Figure 2
Example of air drying GM pellet in a heat block
Figure 3
Figure 3
Wet versus dry GM pellet Left-over ethanol is inhibitory to PCR amplification (left). Completely dried pellet is white and defined (right).
See this image and copyright information in PMC

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