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. 2021 Jun 11;11(34):20865-20873.
doi: 10.1039/d1ra02396a. eCollection 2021 Jun 9.

pH assists simultaneous determination of folic acid and vitamin D3 in biological fluids using a novel Tb3+-acyclovir optical biosensor

Sarah Alharthi  1 , M S Attia  2 , M N Abou-Omar  3
Affiliations

pH assists simultaneous determination of folic acid and vitamin D3 in biological fluids using a novel Tb3+-acyclovir optical biosensor

Sarah Alharthi et al. RSC Adv. .

Abstract

An innovative, simple and cost effective Tb3+-acyclovir photo probe was designed and used as a core for a spectrofluorometric approach to sensitively determine two vital biological compounds in different matrices. The Tb3+-acyclovir complex displays a characteristic electrical band with λ em at 545 nm with significant luminescence intensity, which is quenched in the presence of folic acid and vitamin D3 at pH 5.0 and 9.0, respectively. The conditions were optimized and the best solvent for operation was found to be acetonitrile and λ ex at 340 nm. folic acid was successfully estimated in tablet dosage form, urine and serum in the concentration range of 2.28 × 10-6 to 1.49 × 10-9 mol L-1. Vitamin D3 was also assessed in serum samples using the same optimal conditions within the concentration range of 3.2 × 10-9 to 1.0 × 10-6 mol L -1. The proposed luminescence method was validated in accordance with ICH guidelines and found to be accurate, precise and specific and free from any interferences. The cost effectiveness and applicability of the method make it a good choice for routine analysis of the two compounds and early diagnosis of chronic diseases associated with abnormalities in their physiological levels.

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Conflict of interest statement

There are no conflicts to declare.

Figures

Fig. 1
Fig. 1. Chemical structure of folic acid and vitamin D3.
Fig. 2
Fig. 2. Chemical structure of acyclovir.
Fig. 3
Fig. 3. The absorption spectra of (1) acyclovir (1.0 × 10−4 mol L−1), (2) acyclovir (3.0 × 10−4) + Tb3+ (1.0 × 10−4 mol L−1) and (3) acyclovir (1.0 × 10−4) + Tb3+ (3.0 × 10−4 mol L−1) complex in acetonitrile.
Fig. 4
Fig. 4. The absorption spectrum of (1) ACV, (2) Tb3+–ACV complex, (3) Tb3+–ACV + Vit. D3 and (4) Tb3+–ACV + folic acid in acetonitrile.
Fig. 5
Fig. 5. The emission spectra of Tb3+–ACV complex at λex = 340 nm and pH 5.0 in presence of different folic acid concentrations using acetonitrile as a solvent.
Fig. 6
Fig. 6. The emission spectra of Tb3+–ACV complex at λex = 340 nm and pH 9.0 in presence of different vitamin D3 concentrations using acetonitrile as a solvent.
Fig. 7
Fig. 7. Luminescence emission spectra of Tb3+ with different molar ratio of ACV in acetonitrile at λex = 340 nm and pH 10.0.
Fig. 8
Fig. 8. Emission spectra of Tb3+–ACV optical sensor in different solvents at λex = 340 nm and pH 10.0.
Fig. 9
Fig. 9. Emission spectra of Tb3+–ACV optical sensor in acetonitrile at λex = 340 nm and different pHs.
Fig. 10
Fig. 10. Stern volmer plot (F0/F) − 1 against corresponding concentrations of folic acid.
Fig. 11
Fig. 11. Stern volmer plot (F0/F) − 1 against corresponding concentrations of vitamin D3.
See this image and copyright information in PMC

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