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. 2022 Apr 5;17(1):323-332.
doi: 10.1515/biol-2022-0037. eCollection 2022.

Genetic diversity and population structure of Cinnamomum balansae Lecomte inferred by microsatellites

Bei Cui  1 , Dinh Duy Vu  2   3 , Dinh Giap Vu  4 , Thi Tuyet Xuan Bui  5   6 , Siddiq Ur Rahman  7 , Mai Phuong Pham  3 , Minh Tam Nguyen  8 , Van Sinh Nguyen  5   6 , Syed Noor Muhammad Shah  9 , Viet Ha Tran  10
Affiliations

Genetic diversity and population structure of Cinnamomum balansae Lecomte inferred by microsatellites

Bei Cui et al. Open Life Sci. .

Abstract

Cinnamomum balansae Lecomte (Lauraceae), an economically important forest tree, is distributed in the tropical forests of central and northern Vietnam, which has been threatened in recent decades due to the destruction of its habitat and over-exploitation. The genetic diversity and population structure of the species have not been fully evaluated. We used a set of 15 microsatellites to analyze 161 adult trees from 9 different populations, representing the geographical distribution of C. balansae. Ninety-two different alleles were identified. Here our results showed a low genetic diversity level with an average H o = 0.246 and H e = 0.262, and a high level of genetic differentiation (F ST = 0.601). The bottleneck tests indicated evidence of a reduction in the population size of the two populations (TC and CP). Additionally, all three clustering methods (Bayesian analysis, principal coordinate analysis, and Neighbor-joining tree) were identified in the two genetic groups. The Mantel test showed a significant positive correlation between genetic distance and geographic distance (R 2 = 0.7331). This study will provide a platform for the conservation of C. balansae both in ex-situ and in-situ plans.

Keywords: C. balansae; bottleneck; conservation; fragmentation; genetic variability.

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Conflict of interest statement

Conflict of interest: Authors state no conflict of interest.

Figures

Figure 1
Figure 1
Map showing the collection sites of nine populations of C. balansae in Vietnam.
Figure 2
Figure 2
Mantel test of genetic distance and geographical distance of nine C. balansae populations.
Figure 3
Figure 3
Principal Coordinates (PCoA) (a) and NJ tree for nine populations of C. balansae with bootstrap values above branches (b).
Figure 4
Figure 4
The Delta K distribution graph (a) and bar plot of admixture assignment for nine C. balansae populations to cluster (K = 2 and highest ∆K value = 1145.4) based on Bayesian analysis (b).
See this image and copyright information in PMC

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