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. 2022:2491:3-25.
doi: 10.1007/978-1-0716-2285-8_1.

Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System

Maryam Raeeszadeh-Sarmazdeh  1 , Eric T Boder  2
Affiliations

Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System

Maryam Raeeszadeh-Sarmazdeh et al. Methods Mol Biol. 2022.

Abstract

Yeast surface display has proven to be a powerful tool for the discovery of antibodies and other novel binding proteins and for engineering the affinity and selectivity of existing proteins for their targets. In the decades since the first demonstrations of the approach, the range of yeast display applications has greatly expanded to include many different protein targets and has grown to encompass methods for rapid protein characterization. Here, we briefly summarize the development of yeast display methodologies and highlight several selected examples of recent applications to timely and challenging protein engineering and characterization problems.

Keywords: Antibody engineering; Deep mutational scanning; Directed evolution; In vitro antibody selections; Library screening; Protein engineering; Protein therapeutics; Yeast display.

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