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. 2022:2490:179-193.
doi: 10.1007/978-1-0716-2281-0_13.

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

Federico Pecori #  1 , Hisatoshi Hanamatsu #  2 , Jun-Ichi Furukawa  3 , Shoko Nishihara  4   5
Affiliations

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

Federico Pecori et al. Methods Mol Biol. 2022.

Abstract

Glycosylation is one of the most abundant posttranslational modifications and is involved in a wide range of cellular processes. Glycome diversity in mammals is generated by the action of over 200 distinct glycosyltransferases and related enzymes. Nevertheless, glycosylation dynamics are tightly coordinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.

Keywords: Embryonic stem cells; Epiblast-like cells; Free oligosaccharides; Glycosaminoglycans; Glycosphingolipids; Glycosylation; N-Glycans; O-Glycans; Pluripotent stem cells.

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