Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2490:179-193.
doi: 10.1007/978-1-0716-2281-0_13.

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

Federico Pecori #  1 , Hisatoshi Hanamatsu #  2 , Jun-Ichi Furukawa  3 , Shoko Nishihara  4   5
Affiliations

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

Federico Pecori et al. Methods Mol Biol. 2022.

Abstract

Glycosylation is one of the most abundant posttranslational modifications and is involved in a wide range of cellular processes. Glycome diversity in mammals is generated by the action of over 200 distinct glycosyltransferases and related enzymes. Nevertheless, glycosylation dynamics are tightly coordinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.

Keywords: Embryonic stem cells; Epiblast-like cells; Free oligosaccharides; Glycosaminoglycans; Glycosphingolipids; Glycosylation; N-Glycans; O-Glycans; Pluripotent stem cells.

PubMed Disclaimer

References

    1. Apweiler R, Hermjakob H, Sharon N (1999) On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochim Biophys Acta 1473:4–8. https://doi.org/10.1016/S0304-4165(99)00165-8 - DOI - PubMed
    1. Pecori F, Yokota I, Hanamatsu H, Miura T, Ogura C, Ota H, Furukawa J-I, Oki S, Yamamoto K, Yoshie O, Nishihara S (2021) A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition. Sci Rep 11:1276. https://doi.org/10.1038/s41598-020-79666-4 - DOI - PubMed - PMC
    1. Nishihara S (2018) Glycans in stem cell regulation: from drosophila tissue stem cells to mammalian pluripotent stem cells. FEBS Lett 592:3773–3790. https://doi.org/10.1002/1873-3468.13167 - DOI - PubMed
    1. Varki A (2017) Biological roles of glycans. Glycobiology 27:3–49. https://doi.org/10.1093/glycob/cww086 - DOI - PubMed
    1. Weerapana E, Imperiali B (2006) Asparagine-linked protein glycosylation. Glycobiology 16:91R–101R - DOI

Publication types

LinkOut - more resources