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. 2022 Jun 30;65(8):223-229.
doi: 10.1002/jlcr.3975. Epub 2022 May 6.

Automated synthesis of (R)-[18 F]MH.MZ on the iPhase Flexlab reaction platform

Adam J Rosenberg  1   2 , Yiu-Yin Cheung  1 , Fei Liu  1 , Todd E Peterson  1   2 , James Silverman  3 , Ciaran M Considine  3 , Daniel O Claassen  3
Affiliations

Automated synthesis of (R)-[18 F]MH.MZ on the iPhase Flexlab reaction platform

Adam J Rosenberg et al. J Labelled Comp Radiopharm. .

Abstract

(R)-[18 F]MH.MZ ([18 F]MH.MZ) is a promising positron emission tomography (PET) radiotracer for in vivo study of the 5-HT2A receptor. To facilitate clinical trials, a fully automated radiosynthesis procedure for [18 F]MH.MZ was developed using commercially available materials on the iPhase Flexlab module. The overall synthesis time was 100 min with a radiochemical yield of 7 ± 0.9% (n = 3). The radiochemical purity was greater than 99% for [18 F]MH.MZ with a molar activity of 361 ± 57 GBq/μmol (n = 3). The protocol described herein reliably provides [18 F]MH.MZ that meets all relevant release criteria for a GMP radiopharmaceutical.

Keywords: 5-HT2A receptor; PET; automation; cGMP; fluorine-18; radiochemistry.

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Figures

Figure 1:
Figure 1:
Diagram of the iPhase Flexlab
Figure 2:
Figure 2:
HPLC Chromatograms of reference standard and [18F]MH.MZ
Scheme 1:
Scheme 1:
Synthesis of [18F]MH.MZ
See this image and copyright information in PMC

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