Detection of porcine enteric picornaviruses from faecal samples of Indian pigs

Abstract

Porcine enteric picornaviruses often consequence diarrhoea and nervous complications in pig and pose enormous loss to pig farming. The present study expands the limited Indian data of porcine enteric picornaviruses which is needed for the early implementation of control measures and to check further outbreaks. A total of 398 porcine faecal samples from Uttar Pradesh, Madhya Pradesh, Chhattisgarh and Jharkhand state of India were screened for porcine teschovirus (PTV), porcine sapelovirus (PSV) and enterovirus G (EV-G) by reverse transcriptase-polymerase chain reaction (RT-PCR) using 5'UTR-specific primers. The prevalence of PTV, PSV and EV-G was found to be 12.81% (51/398), 5.77% (23/398) and 24.37% (97/398), respectively. EV-G was relatively higher in circulation in Indian pigs among all the included enteric picornaviruses. Conversely, the concurrent infection of more than one enteric picornavirus was also frequent.

Supplementary information: The online version contains supplementary material available at 10.1007/s13337-022-00756-0.

Keywords: Enterovirus G; India; Pigs; Porcine sapelovirus; Teschovirus.

Fig. 1

Schematic diagram representing areas involved in the sample collection

Fig. 2

Ethidium bromide stained 1.5% agarose gel showing 321 bp amplicons of PTV: Lane 1–5: positive sample, Lane 6: negative test control and Lane M: Marker (100 bp)

Fig. 3

Ethidium bromide stained 1.5% agarose gel showing 211 bp amplicons of PSV: Lane 1–4: positive sample, Lane M: Marker (100 bp) and Lane 5: negative test control

Fig. 4

Ethidium bromide stained 1.5% agarose gel showing 491 bp amplicons of EV-G: Lane 1–3: positive sample, Lane M: Marker (100 bp) and Lane 4: negative test control