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Review
. 2022 Apr 8;25(5):104229.
doi: 10.1016/j.isci.2022.104229. eCollection 2022 May 20.

Hybprinting for musculoskeletal tissue engineering

Jiannan Li  1 , Carolyn Kim  1   2 , Chi-Chun Pan  1   2 , Aaron Babian  3 , Elaine Lui  1   2 , Jeffrey L Young  1 , Seyedsina Moeinzadeh  1 , Sungwoo Kim  1 , Yunzhi Peter Yang  1   4   5
Affiliations
Review

Hybprinting for musculoskeletal tissue engineering

Jiannan Li et al. iScience. .

Abstract

This review presents bioprinting methods, biomaterials, and printing strategies that may be used for composite tissue constructs for musculoskeletal applications. The printing methods discussed include those that are suitable for acellular and cellular components, and the biomaterials include soft and rigid components that are suitable for soft and/or hard tissues. We also present strategies that focus on the integration of cell-laden soft and acellular rigid components under a single printing platform. Given the structural and functional complexity of native musculoskeletal tissue, we envision that hybrid bioprinting, referred to as hybprinting, could provide unprecedented potential by combining different materials and bioprinting techniques to engineer and assemble modular tissues.

Keywords: Biotechnology; biomaterials; materials science; tissue engineering.

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Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
Schematics of 3D printing methods (A) inkjet-based; (B) stereolithography (SLA); (C) extrusion-based printing; (D) selective laser sintering (SLS); (E) fused deposition modeling (FDM); (F) laser-based transfer printing; (G) acoustic droplet ejection.
Figure 2
Figure 2
Recent progress in hybprinting for soft-rigid scaffolds integration (A–C) Integration of FDM with SBM module by (A) (J) Malda (Schuurman et al., 2011), (B) (D) Cho (Shim et al., 2011), and (C) (A) Atala group (Kang et al., 2016). (D) Integration of FDM with SLA by Y.P. Yang group (Shanjani et al., 2015). (Reproduced with the permission from (A, B, D) IOP Publishing and (C) Springer Nature).
Figure 3
Figure 3
Hybprinting logistics and envisioned strategies for musculoskeletal tissue engineering (A) Mechanical property range of different tissues and materials (Bettinger, 2018); (B) Corresponding printing mechanisms for each engineered materials; (C) Schematic of hybprinted vascularized musculoskeletal construct. The grey lattice indicates the bone scaffold printed by FDM, the red conduit indicates the major vascular branches by DLP-SLA, and the space between the grey lattice and red conduits are for the cell-laden hydrogel for angiogenesis and osteogenesis by SBM. (D) By incorporating other printing mechanisms such as inkjet printing, we envision the fabrication of more biomimetic tissue constructs by combining the mechanical gradient and biological gradient. (Reproduced with the permission from (A) Springer Nature).
See this image and copyright information in PMC

References

    1. Agarwala M., Bourell D., Beaman J., Marcus H., Barlow J. Direct selective laser sintering of metals. Rapid Prototyping J. 1995;1:26–36. doi: 10.1108/13552549510078113. - DOI
    1. Ajaxon I., Acciaioli A., Lionello G., Ginebra M.P., Ohman-Magi C., Baleani M., Persson C. Elastic properties and strain-to-crack-initiation of calcium phosphate bone cements: revelations of a high-resolution measurement technique. J. Mech. Behav. Biomed. 2017;74:428–437. doi: 10.1016/j.jmbbm.2017.06.023. - DOI - PubMed
    1. Alabort W., Tang Y.T., Barba D., Reed R.C. Alloys-by-design: a low-modulus titanium alloy for additively manufactured biomedical implants. Acta Materialia. 2022;229
    1. Amr M., Dykes I., Counts M., Kernan J., Mallah A., Mendenhall J., Van Wie B., Abu-Lail N., Gozen A. 3D printed, mechanically tunable, composite sodium alginate, gelatin and Gum Arabic (SA-GEL-GA) scaffolds. Bioprinting. 2021;22
    1. Arcaute K., Mann B., Wicker R. Stereolithography of three-dimensional bioactive poly(ethylene glycol) constructs with encapsulated cells. Ann. Biomed. Eng. 2006;34:1429–1441. doi: 10.1007/s10439-006-9156-y. - DOI - PubMed

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